Specifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ENO3.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, IHC, WB
- APPLICATION NOTE: ENO3 antibody can be used for detection of ENO3 by ELISA at 1:12500. ENO3 antibody can be used for detection of ENO3 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
- POSITIVE CONTROL 1: Cat. No. 1211 - HepG2 Cell Lysate
- PREDICTED MOLECULAR WEIGHT: 47 kDa
Properties
- PURIFICATION: Antibody is purified by protein A chromatography method.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: For short periods of storage (days) store at 4°C. For longer periods of storage, store ENO3 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: ENO3
- ADDITIONAL NAMES: ENO3, MSE, GSD13
- Protein Accession Number: NP_001967
- PROTEIN GI NUMBER: 301897469
- NCBI GENE ID NUMBER: 2027
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- ENO3 is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in ENO3 gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme.This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme. Two transcripts have been identified for this gene that differ only in their 5' UTR.
- 1: Li, T.B., (2004) Acta Biochim. Biophys. Sin. (Shanghai) 36 (6), 412-418.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: This product is for research use only.
Shipping Info
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