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ELK1 Antibody [3H6D12 / 4H9C8 / 4H9F1]
CATALOG NUMBER: 32-148- Clonality: Monoclonal
- Tested Applications: ELISA, IHC, WB
- Host Species: Mouse
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 0.1 mL
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- HOST SPECIES: Mouse
- SPECIES REACTIVITY: Human
- IMMUNOGEN: Ni-NTA purified truncated recombinant ELK1 expressed in E. Coli strain BL21 (DE3).
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, IHC, WB
- APPLICATION NOTE: Western Blot:1:500 - 1:1,000
IHC(P):1:500 - 1:1,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Properties
- CLONALITY: Monoclonal
- ISOTYPE: IgG1
- BUFFER: Ascitic fluid containing 0.03% sodium azide.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: ELK1 monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: ELK1
- ADDITIONAL NAMES: ETS domain-containing protein Elk-1
- Protein Accession Number: P19419
- PROTEIN GI NUMBER: 12643407
- NCBI GENE ID NUMBER: 2002
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- The transcription factor ELK1 is a family of member of ETS oncogene family and of the ternary complex factor (TCF) subfamily,which is located on chromosome Xp11.2 and stimulates transcription. binds to purine-rich DNA sequences. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Elk1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C terminus,It appears to be a direct target of activated MAP kinase. Biochemical studies indicate that Elk1 is a good substrate for MAP kinase, the kinetics of Elk1phosphorylation and activation correlate with MAP kinase activity, and interfering mutants of MAP kinase block Elk1 activation in vivo. More recent studies have shown that Elk1 is also a target of the Stress Activated Kinase SAPK/JNK. Phosphorylation of Elk1 has also been implicated in synaptic plasticity in the adult hippocampus.
- 1: Rao,V.N., et al. 1989.Science.244 (4900):66-70.
- 2: Hsieh,Y.H., et al. 2006.Biochem. Biophys. Res. Commun. 339 (1): 217-225.
- 3: Gille,H., Strahl,T. and Shaw,P.E.1995. Curr. Biol. 5 (10): 1191-1200.
- 4: Gille,H., et al. 1995.EMBO J. 14 (5): 951-962.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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