EIF4G1 Antibody
CATALOG NUMBER: 13-310- Clonality: Polyclonal
- Tested Applications: IF, IHC, IP, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse, Rat
- Conjugate: Unconjugated
- Size: 50 uL
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: Recombinant protein of human EIF4G1
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: IF, IHC, IP, WB
- APPLICATION NOTE: WB: 1:500 - 1:1000
IHC: 1:50 - 1:100
IF: 1:20 - 1:50
IP: 1:20 - 1:50 - POSITIVE CONTROL 1: HeLa
- POSITIVE CONTROL 2: NIH/3T3
- POSITIVE CONTROL 3: PC-12
- PREDICTED MOLECULAR WEIGHT: Observed: 250kDa
Properties
- PURIFICATION: Affinity purification
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: Store at -20°C. Avoid freeze / thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: EIF4G1
- ADDITIONAL NAMES: P220, EIF4F, EIF4G, EIF4GI, PARK18, EIF-4G1, OCT3, OCT4, OTF3,
- NCBI GENE ID NUMBER: 1981
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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