Specifications
- HOST SPECIES: Goat
- SPECIES REACTIVITY: Human, Pig
- HOMOLOGY: Expected Species Reactivity based on sequence homology: Dog, Cow
- IMMUNOGEN: The immunogen for this antibody is: C-SKGNKYSAQGERQ
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, IHC, WB
- APPLICATION NOTE: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 19kDa band observed in lysates of cell lines HepG2 and K562 (calculated MW of 19.8kDa according to NP_004271.1) and in Pig Testis lysates (calculated MW of 19.8kDa according to NP_001230474.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Pancreas. Recommended concentration: 5ug/ml.
- SPECIFICITY: This antibody is expected to recognize both reported isoforms (NP_004271.1; NP_001129122.1).
- PREDICTED MOLECULAR WEIGHT: Approx 19kDa
Properties
- PURIFICATION: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
- CONCENTRATION: 500 ug/mL
- STORAGE CONDITIONS: Aliquot and store at -20°C. Minimize freezing and thawing.
Additional Info
- NCBI OFFICIAL SYMBOL: EEF1E1 / AIMP3 / p18
- ADDITIONAL NAMES: EEF1E1, eukaryotic translation elongation factor 1 epsilon 1, AIMP3, P18, ARS-interacting multifunctional protein 3, aminoacyl tRNA synthetase complex-interacting multifunctional protein 3, eukaryotic translation elongation factor 1 epsilon-1
- Protein Accession Number: NP_004271.1,NP_001129122.1
- PROTEIN GI NUMBER: 4758862
- NCBI GENE ID NUMBER: 9521
Background
- 1: Kwon NH, Kang T, Lee JY, Kim HH, Kim HR, Hong J, Oh YS, Han JM, Ku MJ, Lee SY, Kim S.Dual role of methionyl-tRNA synthetase in the regulation of translation and tumor suppressor activity of aminoacyl-tRNA synthetase-interacting multifunctional protein-3.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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