Dnmt1 Antibody
CATALOG NUMBER: 55-131- Clonality: Polyclonal
- Tested Applications: Flow, WB
- Host Species: Rabbit
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 400 ul
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- IMMUNOGEN: This Dnmt1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1588-1616 amino acids from the C-terminal region of human Dnmt1.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: Flow, WB
- APPLICATION NOTE: For FACS starting dilution is: 1:10~50
For WB starting dilution is: 1:1000 - PREDICTED MOLECULAR WEIGHT: 183 kDa
Properties
- PURIFICATION: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
- CLONALITY: Polyclonal
- ISOTYPE: Rabbit Ig
- PHYSICAL STATE: Liquid
- BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: DNMT1
- ADDITIONAL NAMES: DNA (cytosine-5)-methyltransferase 1, Dnmt1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI, DNA MTase HsaI, MHsaI, MCMT, DNMT1, AIM, CXXC9, DNMT
- Protein Accession Number: P26358
- PROTEIN GI NUMBER: 12231019
- NCBI GENE ID NUMBER: 1786
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2Fbinding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells.
- 1: Peterson, E.J., et al., Cancer Res. 63(20):6579-6582 (2003). Leu, Y.W., et al., Cancer Res. 63(19):6110-6115 (2003). Saito, Y., et al., Int. J. Cancer 105(4):527-532 (2003). Siedlecki, P., et al., Biochem. Biophys. Res. Commun. 306(2):558-563 (2003). Macaluso, M., et al., Oncogene 22(23):3511-3517 (2003).
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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