- Trial Size
DNAJA4 Antibody
CATALOG NUMBER: 8141- Clonality: Polyclonal
- Tested Applications: E, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse, Rat
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (87%)
- IMMUNOGEN: DNAJA4 antibody was raised against a 15 amino acid peptide near the center of human DNAJA4.
The immunogen is located within amino acids 210 - 260 of DNAJA4. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, WB
- APPLICATION NOTE: DNAJA4 antibody can be used for detection of DNAJA4 by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. - SPECIFICITY: DNAJA4 antibody is human, mouse, and rat reactive. At least three isoforms of DNAJA4 are known to exist; this antibody will detect all three isoforms.
- POSITIVE CONTROL 1: Cat. No. 1320 - Human Colon Tissue Lysate
- PREDICTED MOLECULAR WEIGHT: Predicted: 41, 44, 47 kDa
Observed: 40 kDa
Properties
- PURIFICATION: DNAJA4 antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: DNAJA4 antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: DNAJA4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Additional Info
- NCBI OFFICIAL SYMBOL: DNAJA4
- ADDITIONAL NAMES: DnaJ homolog subfamily A member 4, MST104, MSTP104, PRO1472
- Protein Accession Number: NP_061072
- PROTEIN GI NUMBER: 194328758
- NCBI GENE ID NUMBER: 55466
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- The DNAJA4 protein is the mammalian homolog of the chaperone HSP40 (1) and acts as a co-chaperone with the heat shock protein HSP70 (2). DNAJA4 is highly expressed in heart and testis, accounting for approximately 1% of total protein in mouse heart (2). DNAJA4 is also regulated by the sterol regulatory element binding transcription factor 2 (SREBF2) and plays a role in the synthesis of cholesterol (3).
- 1: Ohtsuka K and Hata M. Mammalian HSP40/DNAJ homologs: cloning of novel cDNAs and a proposal for their classification and nomenclature. Cell Stress Chaperones 2000; 5:98-112.
- 2: Abdul KM, Terada K, Gotoh T, et al. Characterization and functional analysis of a heart-enriched DnaJ / Hsp40 homolog dj4/DjA4. Cell Stress Chaperones 2002; 7:156-66.
- 3: Robichon C, Varret M, Le Liepvre X, et al. DnajA4 is a SREBP-regulated chaperone involved in the cholesterol biosynthesis pathway. Biochim. Biophys. Acta 2006; 1761:1107-13.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
$65 Standard Overnight flat rate for as many products shipped within the United States.
$35 Dry Ice Shipment fee may be required on some items.
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