DLL1 Antibody
CATALOG NUMBER: 46-984- Clonality: Polyclonal
- Tested Applications: ELISA, Flow, IF, WB
- Host Species: Goat
- Species Reactivity: Human, Mouse, Rat
- Conjugate: Unconjugated
- Size: 0.1 mg
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- HOST SPECIES: Goat
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: The immunogen for this antibody is: C-ATQRHLTVGEEWSQD
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, Flow, IF, WB
- APPLICATION NOTE: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 75kDa band observed in Mouse Heart and Rat Pancreas lysates (calculated MW of 78.5kDa according to Mouse NP_031891.2 and 77.4kDa according to Rat NP_114452.1). An additional band of nknown identity was also consistently observed at 25kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration:0.3-7ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ membranes of HeLa cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
- POSITIVE CONTROL 1: Cat. No. 1461 - Rat Heart Tissue Lysate
Properties
- PURIFICATION: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
- CONCENTRATION: 500 ug/mL
- STORAGE CONDITIONS: Aliquot and store at -20°C. Minimize freezing and thawing.
Additional Info
- NCBI OFFICIAL SYMBOL: DLL1
- ADDITIONAL NAMES: DLL1, delta-like 1 (Drosophila), DELTA1, DL1, Delta, OTTHUMP00000017690, delta (Drosophila)-like 1, delta-like 1, delta-like 1 protein
- Protein Accession Number: NP_005609.3
- PROTEIN GI NUMBER: 110735443
- NCBI GENE ID NUMBER: 28514
Background
- 1: Ivey KN, Muth A, Arnold J, King FW, Yeh RF, Fish JE, Hsiao EC, Schwartz RJ, Conklin BR, Bernstein HS, Srivastava D. MicroRNA regulation of cell lineages in mouse and human embryonic stem cells. 2008 Mar 2 (3): 219-29.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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