DKK4 Antibody
CATALOG NUMBER: 58-170- Clonality: Polyclonal
- Tested Applications: IHC-P, WB
- Host Species: Rabbit
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 400 ul
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- IMMUNOGEN: This DKK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188-217 amino acids from the C-terminal region of human DKK4.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: IHC-P, WB
- APPLICATION NOTE: For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100 - PREDICTED MOLECULAR WEIGHT: 25 kDa
Properties
- PURIFICATION: This antibody is purified through a protein A column, followed by peptide affinity purification.
- CLONALITY: Polyclonal
- ISOTYPE: Rabbit Ig
- PHYSICAL STATE: Liquid
- BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: DKK4
- ADDITIONAL NAMES: Dickkopf-related protein 4, Dickkopf-4, Dkk-4, hDkk-4, Dickkopf-related protein 4 short form, DKK4
- Protein Accession Number: Q9UBT3
- PROTEIN GI NUMBER: 13124092
- NCBI GENE ID NUMBER: 27121
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- DKK4, like DKK1, DKK2, and DKK3, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis detected no expression of DKK4, but RT-PCR analysis detected DKK4 expression in cerebellum, T-cell, esophagus, and lung cDNA libraries. DKK4 blocks Xenopus Wnt8, Wnt3a, and Wnt2b, but not Dsh or Fz8, induction of a secondary axis in frog embryos, indicating that DKKs antagonize WNT function upstream of WNT receptors.
- 1: Krupnik, V.E., et al., Gene 238(2):301-313 (1999).
- 2: Yoshida, S., et al., Cytogenet. Cell Genet. 94 (1-2), 88-89 (2001).
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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