- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: DcR1 antibody was raised against a peptide corresponding to amino acids in a extracellular domain of human DcR1 precursor.
The immunogen is located within amino acids 140 - 190 of DcR1.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, ICC, IF, WB
- APPLICATION NOTE: DcR1 antibody can be used for detection of DcR1 by Western blot 0.5 μg/mL. An approximate 65 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
- POSITIVE CONTROL 1: Cat. No. 1201 - HeLa Cell Lysate
- POSITIVE CONTROL 2: Cat. No. 17-001 - HeLa Cell Slide
- PREDICTED MOLECULAR WEIGHT: 65 kDa
- PURIFICATION: DcR1 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: DcR1 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/ml
- STORAGE CONDITIONS: DcR1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- NCBI OFFICIAL SYMBOL: TNFRSF10C
- ADDITIONAL NAMES: DcR1 Antibody: LIT, DCR1, TRID, CD263, TRAILR3, TRAIL-R3, DCR1-TNFR, LIT, UNQ321/PRO366, Tumor necrosis factor receptor superfamily member 10C, Decoy TRAIL receptor without death domain, DcR1
- Protein Accession Number: AF012536
- PROTEIN GI NUMBER: 2338421
- NCBI GENE ID NUMBER: 8794
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
- DcR1 Antibody: Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family and induces apoptosis of a variety of tumor cell lines. DR4 and DR5 are the recently identified functional receptors for TRAIL. Two decoy receptors for TRAIL have been identified and designated DcR1/TRID/TRAIL-R3/LIT and DcR2/TRAIL-R4/TRUNDD. DcR1 has extracellular TRAIL-binding domain but lacks intracellular signaling domain. It is a glycophospholipid-anchored cell surface protein. DcR1 transcripts were expressed in many normal human tissues but not in most cancer cell lines. Overexpression of DcR1 did not induce apoptosis, but attenuated TRAIL-induced apoptosis.
- 1: Pan G; O'Rourke K; Chinnaiyan et al.. The receptor for the cytotoxic ligand TRAIL. Science; 1997;276:111-113
- 2: Pan G, Ni J, Wei YF, et al. An antagonist decoy receptor and a death domain-containing receptor for TRAIL. Science 1997;277:815-8
- 3: Sheridan JP, Marsters SA, Pitti RM, et al. A. Control of TRAIL-induced apoptosis by a family of signaling and decoy receptors. Science 1997;277:818-21
- 4: Degli-Esposti MA, Smolak PJ, Walczak H, et al, Smith CA. Cloning and characterization of TRAIL-R3, a novel member of the emerging TRAIL receptor family. J Exp Med 1997;186(7):1165-70
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page.
- Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
CATALOG NUMBER: 2179
- Size: 0.02 mg | 0.1 mg
- List Price: $89.00 – $395.00
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