- Trial Size
DC-SIGN Antibody
CATALOG NUMBER: 2349- Clonality: Polyclonal
- Tested Applications: E, IHC-P, WB
- Host Species: Rabbit
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Mouse: (76%)
- IMMUNOGEN: DC-SIGN antibody was raised against a synthetic peptide corresponding to 17 amino acids near the center of human DC-SIGN.
The immunogen is located within amino acids 250 - 300 of DC-SIGN. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IHC-P, WB
- APPLICATION NOTE: DC-SIGN antibody can be used for detection of DC-SIGN by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. - POSITIVE CONTROL 1: Cat. No. 1309 - Human Placenta Tissue Lysate
- POSITIVE CONTROL 2: Cat. No. 1308 - Human Small Intestine Tissue Lysate
- POSITIVE CONTROL 3: Cat. No. 11-801 - Human Small Intestine Tissue Slide
- PREDICTED MOLECULAR WEIGHT: Predicted: 44 kDa
Observed: 47 kDa
Properties
- PURIFICATION: DC-SIGN Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: DC-SIGN Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: DC-SIGN antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: CD209
- ADDITIONAL NAMES: DC-SIGN Antibody: CDSIGN, CLEC4L, DC-SIGN, DC-SIGN1, CD209 antigen, C-type lectin domain family 4 member L
- Protein Accession Number: Q9NNX6
- PROTEIN GI NUMBER: 46396012
- NCBI GENE ID NUMBER: 30835
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- DC-SIGN Antibody: Dendritic cells (DCs) that control immune responses were recently found to capture and transport HIV from the mucosal area to remote lymph nodes, where DCs hand over HIV to CD4+ T lymphocytes. DCs also amplify the amount of virus and extend the duration of viral infectivity. Multiple strains of HIV-1, HIV-2 and SIV bind to DCs via DC-SIGN. ICAM-3 is the natural ligand for DC-SIGN. A DC-SIGN homologue (termed DC-SIGNR, L-SIGN, and DC-SIGN2) was identified recently. DC-SIGN forms a novel gene family with DC-SIGNR and many alternatively spliced isoforms of DC-SIGN and DC-SIGNR. The expression of DC-SIGN was found in mucosal tissues including placenta, small intestine, and rectum.
- 1: Geijtenbeek TB, Kwon DS, Torensma R, et al. DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans-infection of T cells. Cell 2000;100:587-97.
- 2: Pohlmann S, Baribaud F, Lee B, et al. RW. DC-SIGN interactions with human immunodeficiency virus type 1 and 2 and simian immunodeficiency virus. J. Virol. 2001; 75:4664-72.
- 3: Geijtenbeek TB, Torensma R, van Vliet SJ, et al. Identification of DC-SIGN, a novel dendritic cell-specific ICAM-3 receptor that supports primary immune responses. Cell 2000; 100:575-85.
- 4: Soilleux EJ, Barten R, and Trowsdale J. DC-SIGN; a related gene, DC-SIGNR; and CD23 form a cluster on 19p13. J. Immunol. 2000; 165:2937-42.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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