DARPP 32 (phospho Thr75) Antibody
CATALOG NUMBER: XPS-1005- Clonality: Polyclonal
- Tested Applications: WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse
- Conjugate: Unconjugated
- Size: 0.1 mL
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse
- IMMUNOGEN: DARPP-32 (Thr75) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Thr75 of DARPP-32. The sequence of the immunogen is identical in human and mouse.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: WB
- APPLICATION NOTE: Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. Rabbit anti-DARPP-32 (Thr75) recognizes human and mouse forms of the protein. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
- SPECIFICITY: DARPP 32 antibody is specific for the ~34k DARPP-32 phosphorylated at Thr75.
- PREDICTED MOLECULAR WEIGHT: 32
Properties
- PURIFICATION: Affinity Purified
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20°C.
Additional Info
- NCBI OFFICIAL SYMBOL: Ppp1r1b
- Protein Accession Number: Q6J4I0
- PROTEIN GI NUMBER: 81884861
- NCBI GENE ID NUMBER: 360616
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- DARPP-32, a dopamine (DA) and cAMP-regulated ~32k phosphoprotein, is associated with dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac®.
- 1: Svenningsson, P., Tzavara, E.T., Carruthers, R., Rachleff, I., Wattler, S., Nehls, M., McKinzie, D.L., Fienberg, A.A., Nomikos, G.G.and Greengard, P., "Diverse psychotomimetics act through a common signaling pathway," Science, 302 (2003) 1412 - 1415.
- 2: Lindskog, M., Svenningsson, P., Pozzi, L., Kim, Y., Fienberg, A.A., Bibb, J.A.., Fredholm, B.B., Nairn, A.C., Greengard, P. and Fisone, G., "Involvement of DARPP-32 phosphorylation in the stimulant action of caffeine, Nature (London), 418 (2002) 774 - 778.
- 3: Maldve, R.E., Zhang, T.A., Ferrani-Kile, K., Schreiber, S.S., Lippmann, M.J., Snyder, G.L., Feinberg, A.A., Leslie, S.W., Gonzales, R.A., and Morrisett, R.A., "DARPP-32 and the regulation of the ethanol sensitivity of NMDA receptors in the nucleus accumbens," Nature Neurosci. 5 (2002) 641 - 648.
- 4: Greengard, P., "The neurobiology of slow synaptic transmission," Science 294 (2001) 1024 -1030.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: This product is for research use only.
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