- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CYBB.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, WB
- APPLICATION NOTE: CYBB antibody can be used for detection of CYBB by ELISA at 1:1562500. CYBB antibody can be used for detection of CYBB by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
- POSITIVE CONTROL 1: Cat. No. 1219 - MCF7 Cell Lysate
- PREDICTED MOLECULAR WEIGHT: 63 kDa
- PURIFICATION: Antibody is purified by peptide affinity chromatography method.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: For short periods of storage (days) store at 4°C. For longer periods of storage, store CYBB antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
- NCBI OFFICIAL SYMBOL: CYBB
- ADDITIONAL NAMES: CYBB, CGD, GP91-1, GP91-PHOX, GP91PHOX, NOX2, AMCBX2, p91-PHOX
- Protein Accession Number: NP_000388
- PROTEIN GI NUMBER: 6996021
- NCBI GENE ID NUMBER: 1536
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
- CYBB is a critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. It also functions as a voltage-gated proton channel that mediates the H (+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. Defects in CYBB are a cause of X-linked chronic granulomatous disease (X-CGD).Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
- 1: Fink, K., (2008) J. Immunol. 180 (10), 6911-6922.
- FOR RESEARCH USE ONLY
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- Disclaimer: This product is for research use only.
CATALOG NUMBER: 25-944
- Size: 100 ul
- List Price: $475.00
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