Specifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: Anti-CTRP3 antibody (3565) was raised against a peptide corresponding to 16 amino acids near the center of human CTRP3.
The immunogen is located within amino acids 90-140 of CTRP3. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, IHC-P, WB
- APPLICATION NOTE: WB: 1 μg/mL; IHC: 2 μg/mL; IF: 10 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in mouse samples; Immunofluorescence in mouse samples. All other applications and species not yet tested. - SPECIFICITY: Multiple isoforms of CTRP3 are known to exist. CTRP3 antibody is predicted to not recognize other members of the CTRP protein family.
- POSITIVE CONTROL 1: Cat. No. 1401 - Mouse Heart Tissue Lysate
- POSITIVE CONTROL 2: Cat. No. 1203 - A549 Cell Lysate
- POSITIVE CONTROL 3: Cat. No. 1205 - Jurkat Cell Lysate
- POSITIVE CONTROL 4: Cat. No. 1282 - 3T3/NIH Cell Lysate
- PREDICTED MOLECULAR WEIGHT: Predicted: 27kD
Observed: 30 kD (Post-modyfication: 1 N-linked glycosylation)
Advanced Validation
- VALIDATION:
Recombinant Protein Test (Figure 2): Anti-CTRP3 antibodies (3565) detected human CTRP3 recombinant protein.
- ISOFORMS: Human CTRP3 has 3 isoforms, including isoform 1 (246aa, 27kD), isoform 2 (198aa, 22kD) and isoform 3 (319aa, 35kD). Mouse CTRP3 has one isoform (246aa, 27kD) and Rat CTRP3 also has one isoform (319aa, 35kD). 3565 can detect human, mouse and rat.
Properties
- PURIFICATION: CTRP3 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: CTRP3 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: CTRP3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: C1QTNF3
- ADDITIONAL NAMES: CTRP3 Antibody: CORS, CORCS, CTRP3, CORS26, C1ATNF3, CORS-26, UNQ753/PRO1484, Complement C1q tumor necrosis factor-related protein 3, Collagenous repeat-containing sequence 26 kDa protein
- Protein Accession Number: NP_852100
- PROTEIN GI NUMBER: 62739183
- NCBI GENE ID NUMBER: 114899
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- CTRP3 Antibody: Adipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines. One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-alpha-related proteins (CTRPs) 1-7. Unlike adiponectin, which is expressed exclusively by differentiated adipocytes, the CTRPs are expressed in a wide variety of tissues. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene. It has been suggested that CTRP3 may play a role in skeletal development.
- 1: Fantuzzi. J. Allergy Clin. Immunol. 2005; 115:911-9.
- 2: Tsao et al. Euro. J. Pharmacol. 2002; 440:213-21.
- 3: Wong et al. Proc. Natl. Acad. Sci. USA 2004; 101:10302-7.
- 4: Shapiro and Scherer. Curr. Biol. 1998; 8:335-8.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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