- HOST SPECIES: Mouse
- SPECIES REACTIVITY: Human
- IMMUNOGEN: Mouse monoclonal CTLA-4 antibodies were raised against the extracellular domain of human CTLA-4.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, Flow, ICC, IF, IHC-P, WB
- SET CONTENTS: CTLA-4 Antibody [1E6], Cat. No. RF16011 (20 μg)
CTLA-4 Antibody [2G10], Cat. No. RF16012 (20 μg)
CTLA-4 Antibody [8A1], Cat. No. RF16013 (20 μg)
- POSITIVE CONTROL 1: Cat. No. RF10001-04 - Human Tonsil Tissue Lysate
- POSITIVE CONTROL 2: Cat. No. RF10001-05 - Lymph Node Tissue Lysate
- PURIFICATION: Antibodies are supplied as protein A purified IgG1.
- CLONALITY: Monoclonal
- ISOTYPE: IgG1
- PHYSICAL STATE: Liquid
- BUFFER: PBS containing 0.02% sodium azide.
- CONCENTRATION: Antibody 1 mg/mL
- STORAGE CONDITIONS: Antibodies can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
- The cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), also known as CD152, is a member of the immunoglobulin superfamily that is expressed by activated T cells and transmits an inhibitory signal to T cells (1,2). Both it and the homologous T-cell co-stimulatory protein CD28 bind to CD80 (B7-H1) and CD86 (B7-H2) on antigen-presenting cells (APCs) (3). Mutations in the CTLA-4 gene have been implicated in multiple autoimmune diseases (4). CTLA-4 also functions as an immune checkpoint protein, and anti- CTLA-4 antibodies have been successfully used in the treatment of cancer (5).ProSci's Risk-FreeTM antibodies are mouse monoclonal antibodies made to improve in vivo studies. Unlike antibodies developed using proteins made in yeast or bacteria, Risk-FreeTM antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. Validated for flow cytometry and ELISA Rigorously tested for the following applications: Immunoblot Immunohistochemistry Immunocytochemistry Immunofluorescence Multiple antibodies per target allowing the user to choose the best antibody for their application Available individually or as a set Risk-FreeTM means they are guaranteed to work for their approved applications
- 1: Brunet JF, Denizot F, Luciani MF, et al. A new member of the immunoglobulin superfamily--CTLA-4 . Nature 1987; 328:267–70.
- 2: Walunas TL, Lenschow DJ, Bakker CY, et al. CTLA-4 can function as a negative regulator of T cell activation. Immunity 1994; 1:405–13.
- 3: Harding FA, McArthur JG, Gross JA, et al. CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones. Nature 1992; 356:607–9.
- 4: Romo-Tena J, Gomez-Martin D, and Alcocer-Verela J. CTLA-4 and autoimmunity: new insights into the dual regulator of tolerance. Autoimmun. Rev. 2013; 12:117.
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page.
- Disclaimer: This product is for research use only.
CATALOG NUMBER: RF16010
- Size: 1 Set
- List Price: $210.00
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