Name: CTLA4 Antibody [8A1]
SKU: RF16013
Availability: InStock

CATALOG NUMBER: RF16013

HOST SPECIES: Mouse
SPECIES REACTIVITY: Human
IMMUNOGEN: CTLA-4 antibody was raised against the extracellular domain of human CTLA-4.
CONJUGATE: Unconjugated
TESTED APPLICATIONS: E, ICC, IF, IHC-P, WB
APPLICATION NOTE: CTLA-4 antibody can be used for detection of CTLA-4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
POSITIVE CONTROL 1: Cat. No. 1210 - HEK293 Lysate Cell Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 25 kDa
Observed: 32 kDa

PURIFICATION: CTLA-4 Antibody is supplied as protein A purified IgG1.
CLONALITY: Monoclonal
ISOTYPE: IgG1
PHYSICAL STATE: Liquid
BUFFER: CTLA-4 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
CONCENTRATION: 1 mg/mL
STORAGE CONDITIONS: CTLA-4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

NCBI OFFICIAL SYMBOL: CTLA4
ADDITIONAL NAMES: CTLA-4 Antibody: CTL4, cytotoxic T-lymphocyte associated protein 4, CD, GSE, GRD4, ALPS5, CD152, IDDM12, CELIAC3
Protein Accession Number: NP_005205
NCBI GENE ID NUMBER: 1493
USER NOTE: Optimal dilutions for each application to be determined by the researcher.

The cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), also known as CD152, is a member of the immunoglobulin superfamily that is expressed by activated T cells and transmits an inhibitory signal to T cells (1,2). Both it and the homologous T-cell co-stimulatory protein CD28 bind to CD80 (B7-H1) and CD86 (B7-H2) on antigen-presenting cells (APCs) (3). Mutations in the CTLA-4 gene have been implicated in multiple autoimmune diseases (4). CTLA-4 also functions as an immune checkpoint protein, and anti- CTLA-4 antibodies have been successfully used in the treatment of cancer (5).
1: Brunet JF, Denizot F, Luciani MF, et al. A new member of the immunoglobulin superfamily--CTLA-4 . Nature 1987; 328:267–70.
2: Walunas TL, Lenschow DJ, Bakker CY, et al. CTLA-4 can function as a negative regulator of T cell activation. Immunity 1994; 1:405–13.
3: Harding FA, McArthur JG, Gross JA, et al. CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones. Nature 1992; 356:607–9.
4: Romo-Tena J, Gomez-Martin D, and Alcocer-Verela J. CTLA-4 and autoimmunity: new insights into the dual regulator of tolerance. Autoimmun. Rev. 2013; 12:117.

FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page.
Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER: RF16013

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CATALOG NUMBER: 95-201

CATALOG NUMBER: 95-200

CATALOG NUMBER: 9805

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