Specifications
- SPECIES: Human
- SOURCE SPECIES: HEK293 cells
- RECOMBINANT PROTEIN SEQUENCE: Ala 37 - Phe 162
- FUSION TAG: His Tag
- TESTED APPLICATIONS: WB
- APPLICATION NOTE: This recombinant protein can be used for WB. For research use only.
- PREDICTED MOLECULAR WEIGHT: 14.3 kDa
- BIOLOGICAL ACTIVITY: Measured by its binding ability in a functional ELISA. Immobilized Human CTLA4 Protein at 2μg/mL (100μL/well) can bind rhB7-1/ CD80 Protein Fc Chimera with a linear range of 2 - 50 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized Human CTLA4 Protein at 10μg/mL (100μL/well) can bind rh PD-L1 /B7-H1 /CD274 Protein Fc Chimera with a linear range of 20 - 10000 ng/mL.
Properties
- PURITY: >95% as determined by SDS-PAGE.
- PHYSICAL STATE: Lyophilized
- BUFFER: PBS, pH7.4
- STORAGE CONDITIONS: Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: CTLA4
- ADDITIONAL NAMES: CTLA4, CD152, CELIAC3, GRD4, GSE, ICOS, IDDM12
- Protein Accession Number: NP_005205.2
- NCBI GENE ID NUMBER: 1493
Background
- CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. T cell activation through the T cell receptor and CD28 leads to increased expression of CTLA-4, an inhibitory receptor for B7 molecules. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.
- 1: Waterhouse P, et al., 1995, Science 270 (5238): 985–8.
- 2: Magistrelli G, et al., 1999. Eur. J. Immunol. 29 (11): 3596–602.
- 3: Rudd, CE. et al., 2009, Immunol. Rev. 229 (1): 12-26.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.
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