Specifications
- SPECIES: Human
- SOURCE SPECIES: HEK293 cells
- RECOMBINANT PROTEIN SEQUENCE: Ala 37 - Phe 162
- FUSION TAG: His Tag
- TESTED APPLICATIONS: WB
- APPLICATION NOTE: This recombinant protein can be used for WB. For research use only.
- PREDICTED MOLECULAR WEIGHT: 14.3 kDa
- BIOLOGICAL ACTIVITY: Measured by its binding ability in a functional ELISA. Immobilized Human CTLA4 Protein at 2μg/mL (100μL/well) can bind rhB7-1/ CD80 Protein Fc Chimera with a linear range of 2 - 50 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized Human CTLA4 Protein at 10μg/mL (100μL/well) can bind rh PD-L1 /B7-H1 /CD274 Protein Fc Chimera with a linear range of 20 - 10000 ng/mL.
Properties
- PURITY: >95% as determined by SDS-PAGE.
- PHYSICAL STATE: Lyophilized
- BUFFER: PBS, pH7.4
- STORAGE CONDITIONS: Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: CTLA4
- ADDITIONAL NAMES: CTLA4, CD152, CELIAC3, GRD4, GSE, ICOS, IDDM12
- Protein Accession Number: NP_005205.2
- NCBI GENE ID NUMBER: 1493
Background
- CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. T cell activation through the T cell receptor and CD28 leads to increased expression of CTLA-4, an inhibitory receptor for B7 molecules. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.
- 1: Waterhouse P, et al., 1995, Science 270 (5238): 985–8.
- 2: Magistrelli G, et al., 1999. Eur. J. Immunol. 29 (11): 3596–602.
- 3: Rudd, CE. et al., 2009, Immunol. Rev. 229 (1): 12-26.
Disclaimer
- FOR RESEARCH USE ONLY
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