creatine kinase M-type Antibody
CATALOG NUMBER: 43-272- Clonality: Polyclonal
- Tested Applications: E, IHC, WB
- Host Species: Goat
- Species Reactivity: Human, Mouse
- Conjugate: Unconjugated
- Size: 0.1 mg
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- HOST SPECIES: Goat
- SPECIES REACTIVITY: Human, Mouse
- HOMOLOGY: Expected Species Reactivity based on sequence homology: Dog
- IMMUNOGEN: The immunogen for this antibody is: NTHNKFKLNYKPEE-C
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IHC, WB
- APPLICATION NOTE: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 45kDa band observed in Human and Mouse Skeletal Muscle and Heart lysates (calculated MW of 43.1kDa according to NP_001815.2). Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Heart. Recommended concentration: 5ug/ml.
- PREDICTED MOLECULAR WEIGHT: Approx 45kDa
Properties
- PURIFICATION: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
- CONCENTRATION: 500 ug/mL
- STORAGE CONDITIONS: Aliquot and store at -20°C. Minimize freezing and thawing.
Additional Info
- NCBI OFFICIAL SYMBOL: creatine kinase M-type
- ADDITIONAL NAMES: CKM, creatine kinase, muscle, CKMM, M-CK, creatine kinase M chain, creatine kinase M-type, creatine kinase-M
- Protein Accession Number: NP_001815.2
- PROTEIN GI NUMBER: 21536288
- NCBI GENE ID NUMBER: 1158
Background
- 1: Diguet N, Mallat Y, Ladouce R, Clodic G, Prola A, Tritsch E, Blanc J, Larcher JC, Delcayre C, Samuel JL, Friguet B, Bolbach G, Li Z, Mericskay M.Muscle creatine kinase deficiency triggers both actin depolymerization and desmin disorganization by advanced glycation end products in dilated cardiomyopathy.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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