Specifications
- HOST SPECIES: Goat
- SPECIES REACTIVITY: Human
- IMMUNOGEN: The immunogen for this antibody is: C-DIADLFQVPKAYS
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, IHC
- APPLICATION NOTE: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave no signal but low background in human kidney and HepG2 lysates at up to 1ug/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Human Heart shows staining of myocardial fibres in longitudinal section. Recommended concentration, 3-5ug/ml.
- SPECIFICITY: This antibody is expected to recognise human isoforms a (NP_689452.1; NP_689451.1; NP_004368.1; NP_001138609.1; NP_001138607.1), isoform c (NP_001138606.1) and isoform d (NP_001138608.1).
- POSITIVE CONTROL 1: Cat. No. 1301 - Human Heart Tissue Lysate
- POSITIVE CONTROL 2: Cat. No. 10-501 - Human Heart Tissue Slide
Properties
- PURIFICATION: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
- CONCENTRATION: 500 ug/mL
- STORAGE CONDITIONS: Aliquot and store at -20°C. Minimize freezing and thawing.
Additional Info
- NCBI OFFICIAL SYMBOL: CPT1B
- ADDITIONAL NAMES: CPT1B, carnitine palmitoyltransferase I, muscle, carnitine palmitoyltransferase 1B (muscle) , CPT1-M, M-CPT1, KIAA1670, CPTI, muscle, carnitine O-palmitoyltransferase I, mitochondrial muscle isoform, carnitine palmitoyltransferase 1A (muscle), carnitine palmitoyltransferase 1B, carnitine palmitoyltransferase 1B (muscle), CPT1M, FLJ55729, FLJ58750, MCCPT1, MCPT1, carnitine palmitoyltransferase I-like
- Protein Accession Number: NP_689452.1,NP_689451.1,NP_004368.1,NP_001138609.1,NP_001138607.1,NP_001138606.1,NP_001138608.1
- PROTEIN GI NUMBER: 4758050
- NCBI GENE ID NUMBER: 1375
Background
- 1: Yamazaki N, Shinohara Y, Shima A, Yamanaka Y, Terada H. Isolation and characterization of cDNA and genomic clones encoding human muscle type carnitine palmitoyltransferase I. Biochim Biophys Acta. 1996 Jun 7;1307(2):157-61.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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