- SPECIES: Human
- SOURCE SPECIES: HEK293 cells
- RECOMBINANT PROTEIN SEQUENCE: Gln 19 - Cys 1676
- FUSION TAG: His Tag
- TESTED APPLICATIONS: ELISA, WB
- APPLICATION NOTE: This protein carries a polyhistidine tag at the C-terminus. The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 73.9 kDa (β chain) and 114.3 kDa (α
- PREDICTED MOLECULAR WEIGHT: 73.9 kDa (β chain) & 114.3 kDa (α chain)
- PURITY: >95% as determined by SDS-PAGE.
- PHYSICAL STATE: Lyophilized
- BUFFER: PBS, pH7.4
- STORAGE CONDITIONS: Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
- NCBI OFFICIAL SYMBOL: C5
- ADDITIONAL NAMES: Complement C5,C5,CPAMD4
- Protein Accession Number: NP_001726.2
- NCBI GENE ID NUMBER: 727
- Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain). Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. The C5a anaphylatoxin interacts with C5AR1 and tick complement inhibitor. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
- 1: DeMartino JA, et al. 1994. J Biol Chem. 269(20):14446-50.
- 2: Fredslund F, et al. 2008. Nat Immunol. 9(7):753-60.
- 3: Chen Z, et al. 1998. J Biol Chem. 273(17):10411-9.
- FOR RESEARCH USE ONLY
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CATALOG NUMBER: 11-294
- Size: 0.1 mg
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