- IAV
- Trial Size
cIAP Antibody
CATALOG NUMBER: 3325- Clonality: Polyclonal
- Tested Applications: E, IF, IHC-P, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse, Rat
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Pig: (93%), Chicken: (92%)
- IMMUNOGEN: Anti-cIAP antibody (3325) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human cIAP. c-IAP antibody detects both c-IAP1 and c-IAP2.
The immunogen is located within amino acids 540-590 of cIAP. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, IHC-P, WB
- APPLICATION NOTE: WB: 1-4 μg/mL; IHC: 10 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested. - POSITIVE CONTROL 1: Cat. No. 1331 - Human Lung Tissue Lysate
- POSITIVE CONTROL 2: Cat. No. 1203 - A549 Cell Lysate
- POSITIVE CONTROL 3: Cat. No. 1220 - SK-N-SH Cell Lysate
- PREDICTED MOLECULAR WEIGHT: Predicted: 70kD
Observed: 70kD
Advanced Validation
- VALIDATION:
Independent Antibody Validation in Cell lines (Figure 2) shows similar cIAP expression profile in human, mouse and rat cell lines detected by two independent anti-cIAP antibodies that recognize different epitopes, 3325 against C-terminus domain and competitor antibody against recombinant protein. cIAP proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies.
- ISOFORMS: Human cIAP has 2 isoforms, including isoform 1 (618aa, 70kD) and isoform 2 (569aa, 64kD). Mouse cIAP has one isoform (612aa, 70kD) and Rat cIAP also has one isoform (589aa, 67kD). 3325 can detect human, mouse and rat.
Properties
- PURIFICATION: cIAP Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: cIAP Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: cIAP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: BIRC2
- ADDITIONAL NAMES: cIAP Antibody: API1, MIHB, HIAP2, RNF48, cIAP1, Hiap-2, c-IAP1, API1, IAP2, Baculoviral IAP repeat-containing protein 2, C-IAP1, IAP-2
- Protein Accession Number: NP_001157
- PROTEIN GI NUMBER: 4502141
- NCBI GENE ID NUMBER: 329
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- cIAP Antibody: Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and can be prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. The two isoforms of c-IAP (c-IAP1 and c-IAP2) are structurally related to XIAP, containing 3 baculoviral IAP repeat (BIR) motifs that are essential and sufficient for the binding and inhibition of caspases-3, -7. The c-IAPs can associate with the death receptor TNF-R2, and mediate the ubiquitinization of TRAF2 following the binding of TNF-α by its receptor. Omi, a negative regulator of c-IAP, inhibits its activity by catalytically cleaving c-IAP. Another negative regulator, Smac/DIABLO, acts by enhancing the auto-ubiquitization activity of c-IAP.
- 1: Schimmer. Cancer Res. 2004; 64:7183-90.
- 2: Rothe et al. Cell 1995; 83:1243-52.
- 3: Deveraux et al. EMBO J. 1998; 17:2215-23.
- 4: Li et al. Nature 2002; 416:345-7.
Citations
- 1: Nuutinen et al. The effect of microenvironmental CD40 signals on TRAIL- and drug-induced apoptosis in follicular lymphoma cells. Scand J Immunol. 2009;70(6):565-73.PMID: 19906199
- 2: Hahm and Singh. Withaferin A-induced apoptosis in human breast cancer cells is associated with suppression of inhibitor of apoptosis family protein expression. Cancer Lett. 2013;334(1):101-8. PMID: 22935676
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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