- HOST SPECIES: Goat
- HOMOLOGY: Expected Species Reactivity based on sequence homology: Mouse, Rat
- IMMUNOGEN: The immunogen for this antibody is: C-DSFKWASSTEKQPE
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E
- APPLICATION NOTE: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave bands at approx. 38kDa and 35kDa in Mouse Small Intestine lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 44.5kDa according to NP_064334.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
- PURIFICATION: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
- CONCENTRATION: 500 ug/mL
- STORAGE CONDITIONS: Aliquot and store at -20°C. Minimize freezing and thawing.
- NCBI OFFICIAL SYMBOL: Chst5
- ADDITIONAL NAMES: Chst5, carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 5, AI173964, GST-4, I-GlcNAc-6-ST, I-GlcNAc6ST, N-acetylglucosamine 6-O-sulfotransferase, OTTHUMP00000174950, intestinal GlcNAc-6-sulfotransferase
- Protein Accession Number: NP_064334.1
- PROTEIN GI NUMBER: 9910284
- NCBI GENE ID NUMBER: 56773(mouse);307859(rat);
- 1: Kitayama K, Hayashida Y, Nishida K, Akama TO. Enzymes responsible for synthesis of corneal keratan sulfate glycosaminoglycans. The Journal of biological chemistry 2007 Oct 282 (41): 30085-96.
- FOR RESEARCH USE ONLY
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- Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
CATALOG NUMBER: 42-078
- Size: 0.1 mg
- List Price: $350.00
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