Specifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: Recombinant fusion protein containing a sequence corresponding to amino acids 270-559 of human CHAF1B (NP_005432.1).
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: IF, IHC, WB
- APPLICATION NOTE: WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:20 - 1:100 - POSITIVE CONTROL 1: HeLa
- POSITIVE CONTROL 2: 293T
- POSITIVE CONTROL 3: MCF7
- POSITIVE CONTROL 4: U-251MG
- PREDICTED MOLECULAR WEIGHT: Observed: 70kDa
Properties
- PURIFICATION: Affinity purification
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: Store at -20°C. Avoid freeze / thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: CHAF1B
- ADDITIONAL NAMES: CHAF1B, CAF-1, CAF-IP60, CAF1, CAF1A, CAF1P60, MPHOSPH7, MPP7
- NCBI GENE ID NUMBER: 8208
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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