CDK8 Antibody
CATALOG NUMBER: 31-044- Clonality: Polyclonal
- Tested Applications: E, WB
- Host Species: Rabbit
- Species Reactivity: Dog, Human, Mouse, Rat, Zebrafish
- Conjugate: Unconjugated
- Size: 100 ul
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Dog, Human, Mouse, Rat, Zebrafish
- IMMUNOGEN: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CDK8.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, WB
- APPLICATION NOTE: CDK8 antibody can be used for detection of CDK8 by ELISA at 1:12500. CDK8 antibody can be used for detection of CDK8 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
- POSITIVE CONTROL 1: Cat. No. 1211 - HepG2 Cell Lysate
- PREDICTED MOLECULAR WEIGHT: 53 kDa
Properties
- PURIFICATION: Antibody is purified by protein A chromatography method.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: For short periods of storage (days) store at 4°C. For longer periods of storage, store CDK8 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: CDK8
- ADDITIONAL NAMES: CDK8, K35
- Protein Accession Number: NP_001251
- PROTEIN GI NUMBER: 4502745
- NCBI GENE ID NUMBER: 1024
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- The protein encoded by CDK8 is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit cyclin C are components of the RNA polymerase II holoenzyme complex, which phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. This kinase has also been shown to regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery.
- 1: Akoulitchev, S., et al., (2000) Nature 407 (6800), 102-106.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: This product is for research use only.
Shipping Info
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