CDK4 Antibody
CATALOG NUMBER: 62-965- Clonality: Polyclonal
- Tested Applications: Flow, IF, IHC-P, WB
- Host Species: Rabbit
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 400 ul
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- IMMUNOGEN: This CDK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 273-305 amino acids from the C-terminal region of human CDK4.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: Flow, IF, IHC-P, WB
- APPLICATION NOTE: For IF starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50 - PREDICTED MOLECULAR WEIGHT: 34 kDa
Properties
- PURIFICATION: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
- CLONALITY: Polyclonal
- ISOTYPE: Rabbit Ig
- PHYSICAL STATE: Liquid
- BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: CDK4
- ADDITIONAL NAMES: Cyclin-dependent kinase 4, Cell division protein kinase 4, PSK-J3, CDK4
- Protein Accession Number: P11802
- PROTEIN GI NUMBER: 1168867
- NCBI GENE ID NUMBER: 1019
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- CDK4 is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of the gene have been reported.
- 1: Mori, N., et al., Int. J. Hematol. 77(3):259-262 (2003).
- 2: Masaki, T., et al., Hepatology 37(3):534-543 (2003).
- 3: Gump, J., et al., J. Biol. Chem. 278(9):6619-6622 (2003).
- 4: Ramirez, R.D., et al., Oncogene 22(3):433-444 (2003).
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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