Cdc2 (phospho Tyr15) Antibody
CATALOG NUMBER: XPS-1003- Clonality: Polyclonal
- Tested Applications: WB
- Host Species: Rabbit
- Species Reactivity: Dog, Frog, Human, Mouse, Rat
- Conjugate: Unconjugated
- Size: 0.1 mL
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Dog, Frog, Human, Mouse, Rat
- IMMUNOGEN: Cdc2 (Tyr15) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated-Tyr15 of human, mouse, rat and xenopus cdc2.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: WB
- APPLICATION NOTE: The antibody is purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns. Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Western blots of SK-N-MC total cell extracts with phospho- and non-phosphopeptide competition were used to establish the phospho-specificity of the antibody. Immunolabeling of the Mr 34 kDa cdc2 protein is blocked by the Ser15 phosphopeptide used as antigen but not by the corresponding non-phosphopeptide. Initial recommended range of dilutions: 1:500 to 1:2000. Applications include Dot Blots (DB) and Western Blot (WB). Suitability for Immunohistochemistry (IHC) has not been determined. Drosophila, human, mouse, rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
- SPECIFICITY: Cdc2 antibody is specific for cdc2 phosphorylated at Tyr15.
- PREDICTED MOLECULAR WEIGHT: 34
Properties
- PURIFICATION: Affinity Purified
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: For long term storage –80°C is recommended, but shorter term storage at –20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Additional Info
- NCBI OFFICIAL SYMBOL: Clk2
- Protein Accession Number: Q5XI98
- PROTEIN GI NUMBER: 81910362
- NCBI GENE ID NUMBER: 365842
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Cdc2 is a highly conserved protein serine kinase that plays a key role in regulation of the cell cycle. The ability of cdc2 to exercise control over the cell cycle is dependent upon the phosphorylation of Tyr15 in cdc2.
- 1: Sible, J.C., "Cell biology: thanks for the memory," Nature (London), 426 (2003) 392-393.
- 2: Sagata, N., "Molecular biology: untangling checkpoints," Science, 298 (2002) 1905-1907.
- 3: Nakamizo, A., Inamura, T., Inoha, S., Amano, T., Ochi, H., Ikezaki, K., and Fukui, M., "Suppression of cdc2 dephosphorylation at the tyrosine 15 residue during nitrosourea-induced G2M phase arrest in glioblastoma cell lines," J. Neurooncol., 59 (2002) 7-13.
- 4: Maller, J.L., "Mitotic control," Curr. Opinion. Cell Biology, 3 (1991) 269-275.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: This product is for research use only.
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