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Cdc2 Antibody [CDK1/873]
Cdc2 Antibody [CDK1/873]

Cdc2 Antibody [CDK1/873]

CATALOG NUMBER: 33-513
  • Clonality: Monoclonal
  • Tested Applications: Flow, IF, IHC-P
  • Host Species: Mouse
  • Species Reactivity: Bovine, Human, Mink, Monkey
  • Conjugate: Unconjugated
  • Size: 100 ug

Specifications

  • HOST SPECIES: Mouse
  • SPECIES REACTIVITY: Bovine, Human, Mink, Monkey
  • IMMUNOGEN: Recombinant human protein was used as the immunogen for the Cdc2 antibody.
  • CONJUGATE: Unconjugated
  • TESTED APPLICATIONS: Flow, IF, IHC-P
  • APPLICATION NOTE: Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
    Immunofluorescence: 1-2 ug/ml
    Immunohistochemistry (FFPE): 2-4 ug/ml for 30 min at RT (1)
    Optimal dilution of the Cdc2 antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

  • SPECIFICITY: Does not react with Mouse, Rat, Xenopus, Drosophila, S. pombe, S. cerevisiae

Properties

  • PURIFICATION: Protein G affinity chromatography
  • CLONALITY: Monoclonal
  • ISOTYPE: IgG2a, kappa
  • PHYSICAL STATE: Liquid
  • BUFFER: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
  • CONCENTRATION: 0.2 mg/mL
  • STORAGE CONDITIONS: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.

Additional Info

  • NCBI OFFICIAL SYMBOL: CDK1
  • ADDITIONAL NAMES: Cyclin-dependent kinase 1, CDK1, Cell division control protein 2 homolog, Cell division protein kinase 1, p34 protein kinase, CDK1, CDC2, CDC28A, CDKN1, P34CDC2
  • NCBI GENE ID NUMBER: 983
  • USER NOTE: Optimal dilutions for each application to be determined by the researcher

Background

  • Recognizes a 34kDa protein, which is identified as cyclin dependent kinase 1 (cdk1) or p34cdc2 protein kinase. Cdc2 plays a crucial role during cell division and is most active during mitosis. It is predominantly localized in the nucleus. It is a serine/threonine kinase, which is activated by cyclin, presumably by de-phosphorylation of tyrosine residues. Activated Cdc2 performs specific functions during mitosis, including nuclear envelope breakdown and chromosome condensation.

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER: 33-513

  • Size: 100 ug
  • List Price: $535.00
Datasheet
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