- HOST SPECIES: Rat
- SPECIES REACTIVITY: Mouse
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: Flow, Functional Assays, IHC
- SPECIFICITY: The GL-1 monoclonal antibody reacts specifically with the mouse B7-2 (also known as CD86), a costimulatory molecule expressed by B and T lymphocytes, macrophages (thioglycollate-induced from peritoneum), astrocytes and dendritic cells.
- PURIFICATION: The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.
- CLONALITY: Monoclonal
- ISOTYPE: Rat IgG2a, kappa
- PHYSICAL STATE: liquid
- BUFFER: Phosphate-buffered aqueous solution, ph7.2.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: The product should be stored undiluted at 4°C . Do not freeze.
- NCBI OFFICIAL SYMBOL: Cd86
- ADDITIONAL NAMES: B7, B70, MB7, B7-2, B7.2, CLS1, Ly58, ETC-1, Ly-58, MB7-2, Cd28l2, TS/A-2, Cd86
- NCBI GENE ID NUMBER: 12524
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
- The GL-1 monoclonal antibody reacts specifically with the mouse B7-2 (also known as CD86), a costimulatory molecule expressed by B and T lymphocytes, macrophages (thioglycollate-induced from peritoneum), astrocytes and dendritic cells. The memory CD4+ T lymphocytes express CD86 (as mRNA and protein). CD86, and the B7-1 (CD 80) molecule, are ligands for CD152 and CD28, and influence the costimulatory interactions between lymphocytes B and T. B7-2 is also involved in the mouse natural killer cell-mediated cytotoxicity. The GL-1 antibody blocks the mixed lymphocyte reaction (MLR) and inhibits the T-cells stimulation by antigen-presenting cells. Mixtures of anti-B7-1 antibody and GL-1 were reported to inhibit the interaction between CD125 and its ligand with the in vivo priming of cytotoxic T lymphocytes.
- 1: Hathcock, K. S., Laszlo, G., Dickler, H. B., Bradshaw, J., Linsley, P., Hodes, R. J. (1993). Identification of an alternative CTLA-4 ligand costimulatory for T cell activation.Science,262(5135), 905-907.
- 2: Inaba, K., Witmer-Pack, M., Inaba, M., Hathcock, K. S., Sakuta, H., Azuma, M., ... Steinman, R. M. (1994). The tissue distribution of the B7-2 costimulator in mice: abundant expression on dendritic cells in situ and during maturation in vitro.The Journal of experimental medicine,180(5), 1849-1860.
- 3: Hathcock, K. S., Laszlo, G., Pucillo, C., Linsley, P., Hodes, R. J. (1994). Comparative analysis of B7-1 and B7-2 costimulatory ligands: expression and function.The Journal of experimental medicine,180(2), 631-640.
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page.
- Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
CATALOG NUMBER: 76-598
- Size: 0.1 mg
- List Price: $220.00
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