- Trial Size
CD86 Antibody
CATALOG NUMBER: 8681- Clonality: Polyclonal
- Tested Applications: E, IF, IHC-P, WB
- Host Species: Rabbit
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rabbit: (75%), Mouse: (75%)
- IMMUNOGEN: CD86 antibody was raised against a peptide corresponding to 17 amino acids near the center of human CD86.
The immunogen is located within amino acids 160 - 210 of CD86. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, IHC-P, WB
- APPLICATION NOTE: CD86 antibody can be used for Western blot at 1 - 2 μg/mL. Antibody can also be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. - SPECIFICITY: At least five isoforms of CD86 are known to exist; this antibody will detect all five isoforms.
- POSITIVE CONTROL 1: Cat. No. 1224 - Daudi Cell Lysate
- POSITIVE CONTROL 2: Cat. No. 1207 - Raji Cell Lysate
- PREDICTED MOLECULAR WEIGHT: Predicted: 36 kDa
Observed: 68 kDa
Properties
- PURIFICATION: CD86 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: CD86 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: CD86 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: CD86
- ADDITIONAL NAMES: CD86 Antibody: CD86 molecule, B70, B7-2, B7.2, LAB72, CD28LG2
- Protein Accession Number: NP_787058
- PROTEIN GI NUMBER: 332634934
- NCBI GENE ID NUMBER: 942
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- CD86, also known as B7-2, is a type I membrane protein that is a member of the immunoglobulin superfamily. Like the related protein CD80, this protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells, CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4). Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production. CTLA-4 binding negatively regulates T-cell activation and diminishes the immune response (1). Blocking the CTLA-4-CD80/CD86 interaction has been shown to enhance T-cell functions in acute lymphoblastomic leukemia (ALL), suggesting that this pathway may be an attractive target for future cancer immunotherapy (2).
- 1: Lane P. Regulation of T and B cell responses by modulating interactions between CD28/CTLA-4 and their ligands, CD80 and CD86. Ann NY Acad Sci 1997; 815:392-400.
- 2: Feucht J, Kayser S, Gorodezki D, et al. T-cell responses against CD19+ pediatric acute lymphoblastic leukemia mediated by bispecific T-cell engager (BiTE) are regulated contrarily by PD-L1 and CD80/CD86 on leukemic blasts. Oncotarget 2016; 7:76902-19.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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