Specifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (95%)
- IMMUNOGEN: CD81 antibody was raised against a 20 amino acid synthetic peptide near the amino terminus of human CD81.
The immunogen is located within amino acids 30 - 80 of CD81. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, IHC-P, WB
- APPLICATION NOTE: CD81 antibody can be used for detection of CD81 by Western blot at 2.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. - POSITIVE CONTROL 1: Cat. No. 1283 - RAW 264.7 Cell Lysate
- POSITIVE CONTROL 2: Cat. No. 10-101 - Human Lung Tissue Slide
- POSITIVE CONTROL 3: Cat. No. 17-003 - A549 Cell Slide
- PREDICTED MOLECULAR WEIGHT: Predicted: 26 kDa
Observed: 25 kDa
Properties
- PURIFICATION: CD81 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: CD81 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: CD81 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: CD81
- ADDITIONAL NAMES: CD81 Antibody: S5.7, CVID6, TAPA1, TSPAN28, CD81 antigen, 26 kDa cell surface protein TAPA-1, Tspan-28
- Protein Accession Number: NP_004347
- PROTEIN GI NUMBER: 4757944
- NCBI GENE ID NUMBER: 975
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- CD81 Antibody: CD81 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family, a group of cell-surface proteins that are characterized by the presence of four hydrophobic domains and mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD81 is a cell surface glycoprotein that associates with integrins and appears to promote muscle cell fusion and support myotube maintenance. Along with SCARB1, LDL-R, and CLDN1, CD81 has been reported to be an entry factor for the Hepatitis C virus. Finally, recent evidence indicates that the CD81 gene is localized in a tumor-suppressor gene region and is thus a candidate gene for malignancies.
- 1: Oren R, Takahashi S, Doss C, et al. TAPA-1, the target of an antiproliferative antibody, defines a new family of transmembrane proteins. Mol. Cell Biol. 1990; 10:4007-15.
- 2: Charrin S, le Naour F, Silvie O, et al. Lateral organization of membrane proteins: tetraspanins spin their web. Biochem. J. 2009; 420:133-54.
- 3: Mannion BA, Berditchevski F, Kraeft SK, et al. Transmembrane-4 superfamily proteins CD81 (TAPA-1), CD82, CD63, and CD53 specifically associated with integrin alpha 4 beta 1 (CD49d/CD29). J. Immunol. 1996; 157:2039-47.
- 4: Tacvhibana I and Hemler ME. Role of transmembrane 4 superfamily (TM4SF) proteins CD9 and CD81 in muscle cell fusion and myotube maintenance. J. Cell Biol. 1999; 146:893-90.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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