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Home / Flow / CD81 Antibody [1.3.3.22]
CD81 Antibody [1.3.3.22]CD81 Antibody [1.3.3.22]
CD81 Antibody [1.3.3.22]
CD81 Antibody [1.3.3.22]

CD81 Antibody [1.3.3.22]

CATALOG NUMBER: 33-829
  • Clonality: Monoclonal
  • Tested Applications: Flow, Functional Assays, IF, IHC-P, WB
  • Host Species: Mouse
  • Species Reactivity: Human, Mouse, Rat
  • Conjugate: Unconjugated
  • Size: 100 ug

Specifications

  • HOST SPECIES: Mouse
  • SPECIES REACTIVITY: Human, Mouse, Rat
  • IMMUNOGEN: A B-cell line derived from a Burkitt lymphoma was used as the immunogen for the CD81 antibody.
  • CONJUGATE: Unconjugated
  • TESTED APPLICATIONS: Flow, Functional Assays, IF, IHC-P, WB
  • APPLICATION NOTE: Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
    Immunofluorescence: 0.5-1 ug/ml
    WB: 0.5-1 ug/ml
    Functional Studies: order BSA free formulation
    IHC (FFPE): 0.5-1 ug/ml for 30 min at RT
    Optimal dilution of the CD81 antibody should be determined by the researcher.

Properties

  • PURIFICATION: Protein G affinity chromatography
  • CLONALITY: Monoclonal
  • ISOTYPE: IgG1, kappa
  • PHYSICAL STATE: Liquid
  • BUFFER: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
  • CONCENTRATION: 0.2 mg/mL
  • STORAGE CONDITIONS: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.

Additional Info

  • NCBI OFFICIAL SYMBOL: CD81
  • ADDITIONAL NAMES: CD81 Antibody: S5.7, CVID6, TAPA1, TSPAN28, CD81 antigen, 26 kDa cell surface protein TAPA-1, Tspan-28
  • NCBI GENE ID NUMBER: 975
  • USER NOTE: Optimal dilutions for each application to be determined by the researcher

Background

  • This mAb recognizes a protein of 26kDa, identified as CD81 (Workshop VI; Code CD81.1). CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspans transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER: 33-829

  • Size: 100 ug
  • List Price: $555.00
Datasheet
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