Name: CD80 Detection Set (Risk Free)
SKU: RF16040
Price: 365.00 USD
Availability: InStock

CD80 Detection Set (Risk Free)

CATALOG NUMBER: RF16040

Clonality: Monoclonal
Tested Applications: ELISA, Flow, ICC, IF, IHC-P, WB
Host Species: Mouse
Species Reactivity: Human
Conjugate: Unconjugated
Size: 1 Set

Specifications

HOST SPECIES: Mouse
SPECIES REACTIVITY: Human
IMMUNOGEN: CD80 antibodies were raised against the extracellular domain of human CD80.
CONJUGATE: Unconjugated
TESTED APPLICATIONS: ELISA, Flow, ICC, IF, IHC-P, WB
SET CONTENTS: CD80 Antibody [8G12], Cat. No. RF16041 (20 μg)

CD80 Antibody [10A1], Cat. No. RF16042 (20 μg)

CD80 Antibody [7A2], Cat. No. RF16043 (20 μg)

CD80 Antibody [11D1], Cat. No. RF16044 (20 μg)

CD80 Antibody [11C12], Cat. No. RF16045 (20 μg)

CD80 Antibody [12D9], Cat. No. RF16046 (20 μg)
POSITIVE CONTROL 1: Cat. No. RF10001-06 - Raji Lysate
POSITIVE CONTROL 2: Cat. No. RF10001-04 - Human Tonsil Tissue Lysate

Properties

PURIFICATION: Antibodies are supplied as protein A purified IgG1 and IgG2b.
CLONALITY: Monoclonal
ISOTYPE: IgG1, IgG2b
PHYSICAL STATE: Liquid
BUFFER: PBS containing 0.02% sodium azide.
CONCENTRATION: Antibody 1 mg/mL
STORAGE CONDITIONS: Antibodies can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background

CD80, also known as B7-1, is a type I membrane protein that is a member of the immunoglobulin superfamily. Like the related protein CD86, this protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells, CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4). Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production. CTLA-4 binding negatively regulates T-cell activation and diminishes the immune response (1). Blocking the CTLA-4-CD80/CD86 interaction has been shown to enhance T-cell functions in acute lymphoblastomic leukemia (ALL), suggesting that this pathway may be an attractive target for future cancer immunotherapy (2).ProSci's Risk-FreeTM antibodies are mouse monoclonal antibodies made to improve in vivo studies. Unlike antibodies developed using proteins made in yeast or bacteria, Risk-FreeTM antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. Validated for flow cytometry and ELISA Rigorously tested for the following applications: Immunoblot Immunohistochemistry Immunocytochemistry Immunofluorescence Multiple antibodies per target allowing the user to choose the best antibody for their application Available individually or as a set Risk-FreeTM means they are guaranteed to work for their approved applications
1: Lane P. Regulation of T and B cell responses by modulating interactions between CD28/CTLA-4 and their ligands, CD80 and CD86. Ann NY Acad Sci 1997; 815:392-400.
2: Feucht J, Kayser S, Gorodezki D, et al. T-cell responses against CD19+ pediatric acute lymphoblastic leukemia mediated by bispecific T-cell engager (BiTE) are regulated contrarily by PD-L1 and CD80/CD86 on leukemic blasts. Oncotarget 2016; 7:76902-19.

Set Contents

Catalog Number RF16041: CD80 Antibody [8G12]
Catalog Number RF16042: CD80 Antibody [10A1]
Catalog Number RF16043: CD80 Antibody [7A2]
Catalog Number RF16044: CD80 Antibody [11D1]
Catalog Number RF16045: CD80 Antibody [11C12]
Catalog Number RF16046: CD80 Antibody [12D9]

Disclaimer

FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page.
Disclaimer: This product is for research use only.

CATALOG NUMBER: RF16040

Size: 1 Set
List Price: $365.00

Datasheet

Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

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