- HOST SPECIES: Mouse
- SPECIES REACTIVITY: Human
- IMMUNOGEN: CD80 antibodies were raised against the extracellular domain of human CD80.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, Flow, ICC, IF, IHC-P, WB
- SET CONTENTS: CD80 Antibody [8G12], Cat. No. RF16041 (20 μg)
CD80 Antibody [10A1], Cat. No. RF16042 (20 μg)
CD80 Antibody [7A2], Cat. No. RF16043 (20 μg)
CD80 Antibody [11D1], Cat. No. RF16044 (20 μg)
CD80 Antibody [11C12], Cat. No. RF16045 (20 μg)
CD80 Antibody [12D9], Cat. No. RF16046 (20 μg)
- POSITIVE CONTROL 1: Cat. No. RF10001-06 - Raji Lysate
- POSITIVE CONTROL 2: Cat. No. RF10001-04 - Human Tonsil Tissue Lysate
- PURIFICATION: Antibodies are supplied as protein A purified IgG1 and IgG2b.
- CLONALITY: Monoclonal
- ISOTYPE: IgG1, IgG2b
- PHYSICAL STATE: Liquid
- BUFFER: PBS containing 0.02% sodium azide.
- CONCENTRATION: Antibody 1 mg/mL
- STORAGE CONDITIONS: Antibodies can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
- CD80, also known as B7-1, is a type I membrane protein that is a member of the immunoglobulin superfamily. Like the related protein CD86, this protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells, CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4). Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production. CTLA-4 binding negatively regulates T-cell activation and diminishes the immune response (1). Blocking the CTLA-4-CD80/CD86 interaction has been shown to enhance T-cell functions in acute lymphoblastomic leukemia (ALL), suggesting that this pathway may be an attractive target for future cancer immunotherapy (2).ProSci's Risk-FreeTM antibodies are mouse monoclonal antibodies made to improve in vivo studies. Unlike antibodies developed using proteins made in yeast or bacteria, Risk-FreeTM antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. Validated for flow cytometry and ELISA Rigorously tested for the following applications: Immunoblot Immunohistochemistry Immunocytochemistry Immunofluorescence Multiple antibodies per target allowing the user to choose the best antibody for their application Available individually or as a set Risk-FreeTM means they are guaranteed to work for their approved applications
- 1: Lane P. Regulation of T and B cell responses by modulating interactions between CD28/CTLA-4 and their ligands, CD80 and CD86. Ann NY Acad Sci 1997; 815:392-400.
- 2: Feucht J, Kayser S, Gorodezki D, et al. T-cell responses against CD19+ pediatric acute lymphoblastic leukemia mediated by bispecific T-cell engager (BiTE) are regulated contrarily by PD-L1 and CD80/CD86 on leukemic blasts. Oncotarget 2016; 7:76902-19.
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page.
- Disclaimer: This product is for research use only.
CATALOG NUMBER: RF16040
- Size: 1 Set
- List Price: $365.00
$65 Standard Overnight flat rate for as many products shipped within the United States.
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