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CD80 Antibody [8G12]
CD80 Antibody [8G12]
CD80 Antibody [8G12]
CD80 Antibody [8G12]
CD80 Antibody [8G12]
CD80 Antibody [8G12]
CD80 Antibody [8G12]
  • Trial Size

CD80 Antibody [8G12]

CATALOG NUMBER: RF16041
  • Clonality: Monoclonal
  • Tested Applications: ELISA, Flow, ICC, IF, IHC-P
  • Host Species: Mouse
  • Species Reactivity: Human
  • Conjugate: Unconjugated
  • Size: 0.02 mg | 0.1 mg

Specifications

  • HOST SPECIES: Mouse
  • SPECIES REACTIVITY: Human
  • IMMUNOGEN: CD80 antibody was raised against the extracellular domain of human CD80.
  • CONJUGATE: Unconjugated
  • TESTED APPLICATIONS: ELISA, Flow, ICC, IF, IHC-P
  • APPLICATION NOTE: CD80 antibody can be used for immunohistochemistry starting at 2 - 5 μg/mL. For immunofluorescence start at 5 μg/mL. Flow cytometry at 10 μg/ml.
    Antibody validated: Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.
  • POSITIVE CONTROL 1: Cat. No. 1340 - Human Stomach Carcinoma Tissue Lysate
  • POSITIVE CONTROL 2: Cat. No. 1315 - Human Tonsil Tissue
  • PREDICTED MOLECULAR WEIGHT: Predicted: 32 kDa
    Observed: 50 kDa

Properties

  • PURIFICATION: CD80 Antibody is supplied as protein A purified IgG2b.
  • CLONALITY: Monoclonal
  • ISOTYPE: IgG2b
  • PHYSICAL STATE: Liquid
  • BUFFER: CD80 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
  • CONCENTRATION: 1 mg/mL
  • STORAGE CONDITIONS: CD80 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

  • NCBI OFFICIAL SYMBOL: CD80
  • ADDITIONAL NAMES: CD80 Antibody: CD80 molecule, B7, BB1, B7-1, B7.1, LAB7, CD28LG, CD28LG1
  • Protein Accession Number: NP_005182
  • PROTEIN GI NUMBER: 4885123
  • NCBI GENE ID NUMBER: 941
  • USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background

  • CD80 Antibody: CD80, also known as B7-1, is a type I membrane protein that is a member of the immunoglobulin superfamily. Like the related protein CD86, this protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells, CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4). Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production. CTLA-4 binding negatively regulates T-cell activation and diminishes the immune response (1). Blocking the CTLA-4-CD80/CD86 interaction has been shown to enhance T-cell functions in acute lymphoblastomic leukemia (ALL), suggesting that this pathway may be an attractive target for future cancer immunotherapy (2).
  • 1: Lane P. Regulation of T and B cell responses by modulating interactions between CD28/CTLA-4 and their ligands, CD80 and CD86. Ann NY Acad Sci 1997; 815:392-400.
  • 2: Feucht J, Kayser S, Gorodezki D, et al. T-cell responses against CD19+ pediatric acute lymphoblastic leukemia mediated by bispecific T-cell engager (BiTE) are regulated contrarily by PD-L1 and CD80/CD86 on leukemic blasts. Oncotarget 2016; 7:76902-19.

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER: RF16041

  • Size: 0.02 mg | 0.1 mg
  • List Price: $89.00 – $459.00
Datasheet
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