- HOST SPECIES: Rat
- SPECIES REACTIVITY: Mouse
- CONJUGATE: FITC
- TESTED APPLICATIONS: Flow
- SPECIFICITY: The MEL-14 monoclonal antibody specifically reacts with L- selectin (CD62L), a receptor with lectin-like and Epidermal Growth Factor-like domains.
- PURIFICATION: The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
- CLONALITY: Monoclonal
- ISOTYPE: Rat IgG2a, kappa
- PHYSICAL STATE: liquid
- BUFFER: Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze.
- NCBI OFFICIAL SYMBOL: Sell
- ADDITIONAL NAMES: Lnhr, CD62L, Ly-22, Lyam1, Ly-m22, Lyam-1, LECAM-1, AI528707, L-selectin, Sell
- NCBI GENE ID NUMBER: 20343
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
- The MEL-14 monoclonal antibody specifically reacts with L- selectin (CD62L), a receptor with lectin-like and Epidermal Growth Factor-like domains. The weight of the CD62L molecules depend on their origin, 74 kDa on lymphocytes or 95 kDa on neutrophils. In the mouse organism, CD62L can be expressed by most thymocytes, on subsets of B and T lymphocytes, monocytes, eosinophils, and neutrophils. The L-selectin binds sulfated, fucosylated, and glycosylated glycoproteins (MadCAM-1, GLYCAM-1, and CD 34). It mediates the migration of lymphocytes to the site of inflammation and their return to the peripheral lymphoid tissues and to the HEV (high endothelial venules). In vitro, L-selectin inhibits this binding and the lymphocyte extravasation into peripheral lymph nodes. After cell activation, it is quickly shed by neutrophils and lymphocytes.
- 1: Gallatin, W. M., Weissman, I. L., Butcher, E. C. (1982). A cell-surface molecule involved in organ-specific homing of lymphocytes.Nature,304(5921), 30-34.
- 2: Siegelman, M. H., Cheng, I. C., Weissman, I. L., Wakeland, E. K. (1990). The mouse lymph node homing receptor is identical with the lymphocyte cell surface marker Ly-22: role of the EGF domain in endothelial binding.Cell,61(4), 611-622.
- 3: Pizcueta, P., Luscinskas, F. W. (1994). Monoclonal antibody blockade of L-selectin inhibits mononuclear leukocyte recruitment to inflammatory sites in vivo.The American journal of pathology,145(2), 461.
- FOR RESEARCH USE ONLY
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- Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
CATALOG NUMBER: 76-200
- Size: 0.1 mg
- List Price: $245.00
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