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CD5 Antibody [CD5/54/F6]CD5 Antibody [CD5/54/F6]CD5 Antibody [CD5/54/F6]
CD5 Antibody [CD5/54/F6]
CD5 Antibody [CD5/54/F6]
CD5 Antibody [CD5/54/F6]

CD5 Antibody [CD5/54/F6]

CATALOG NUMBER: 33-550
  • Clonality: Monoclonal
  • Tested Applications: Flow, IF, IHC, WB
  • Host Species: Mouse
  • Species Reactivity: Bovine, Chicken, Guinea Pig, Horse, Human, Monkey, Pig, Rabbit, Rat
  • Conjugate: Unconjugated
  • Size: 100 ug

Specifications

  • HOST SPECIES: Mouse
  • SPECIES REACTIVITY: Bovine, Chicken, Guinea Pig, Horse, Human, Monkey, Pig, Rabbit, Rat
  • IMMUNOGEN: A peptide from the intracellular region of the human protein was used as the immunogen for this CD5 antibody.
  • CONJUGATE: Unconjugated
  • TESTED APPLICATIONS: Flow, IF, IHC, WB
  • APPLICATION NOTE: Western blot: 1-2 ug/ml
    Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
    Immunofluorescence: 0.5-1 ug/ml
    Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
    Prediluted format: incubate for 30 min at RT (2)
    The optimal dilution of the CD5 antibody for each application should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Properties

  • PURIFICATION: Protein G affinity chromatography
  • CLONALITY: Monoclonal
  • ISOTYPE: IgG1, kappa
  • PHYSICAL STATE: Liquid
  • BUFFER: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
  • CONCENTRATION: 0.2 mg/mL
  • STORAGE CONDITIONS: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.

Additional Info

  • NCBI OFFICIAL SYMBOL: CD5
  • ADDITIONAL NAMES: T-cell surface glycoprotein CD5, Lymphocyte antigen T1/Leu-1, CD5, CD5, LEU1
  • NCBI GENE ID NUMBER: 921
  • USER NOTE: Optimal dilutions for each application to be determined by the researcher

Background

  • Recognizes a 67kDa transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma (+) from thymoma (-). Anti-CD5 does not react with granulocytes or monocytes.

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER: 33-550

  • Size: 100 ug
  • List Price: $555.00
Datasheet
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