CD47 Antibody
CATALOG NUMBER: 43-581- Clonality: Polyclonal
- Tested Applications: ELISA, WB
- Host Species: Goat
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 0.1 mg
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- HOST SPECIES: Goat
- SPECIES REACTIVITY: Human
- HOMOLOGY: Expected Species Reactivity based on sequence homology: Dog
- IMMUNOGEN: The immunogen for this antibody is: C-SNQKTIQPPRK
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, WB
- APPLICATION NOTE: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 50kDa band observed in Human Hippocampus lysates and 60kDa in lysates of cell lines HeLa and Jurkat (calculated MW of 35.2kDa according to NP_001768.1). The observed molecular weights correspond to glycosylated forms. Recommended concentration: 0.3-1ug/ml.
- SPECIFICITY: This antibody is expected to recognize both reported isoforms (NP_001768.1; NP_942088.1).
- PREDICTED MOLECULAR WEIGHT: Approx 50kDa
Properties
- PURIFICATION: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
- CONCENTRATION: 500 ug/mL
- STORAGE CONDITIONS: Aliquot and store at -20°C. Minimize freezing and thawing.
Additional Info
- NCBI OFFICIAL SYMBOL: CD47
- ADDITIONAL NAMES: CD47, CD47 molecule, IAP, MER6, OA3, CD47 antigen (Rh-related antigen, integrin-associated signal transducer), CD47 glycoprotein, Rh-related antigen, antigen identified by monoclonal antibody 1D8, antigenic surface determinant protein OA3, integrin associ
- Protein Accession Number: NP_001768.1,NP_942088.1
- PROTEIN GI NUMBER: 4502673
- NCBI GENE ID NUMBER: 961
Background
- 1: Azcutia V, Stefanidakis M, Tsuboi N, Mayadas T, Croce KJ, Fukuda D, Aikawa M, Newton G, Luscinskas FW.Endothelial CD47 promotes vascular endothelial-cadherin tyrosine phosphorylation and participates in T cell recruitment at sites of inflammation in vivo.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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