CD3e Antibody
CATALOG NUMBER: 33-082- Clonality: Polyclonal
- Tested Applications: Flow, IP, WB
- Host Species: Rabbit
- Species Reactivity: Bovine, Cat, Chicken, Dog, Elephant, Frog, Guinea Pig, Hamster, Horse, Human, Mole Rat, Mouse, Opossum, Pig, Primate, Rabbit, Rat, Sheep
- Conjugate: Unconjugated
- Size: 100 ug
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Bovine, Cat, Chicken, Dog, Elephant, Frog, Guinea Pig, Hamster, Horse, Human, Mole Rat, Mouse, Opossum, Pig, Primate, Rabbit, Rat, Sheep
- IMMUNOGEN: Amino acids ERPPPVPNPDYEP (156-168) were used as the immunogen for this CD3e antibody.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: Flow, IP, WB
- APPLICATION NOTE: Flow Cytometry: use the CD3e antibody at 0.5-1 ug/million cells
WB: 0.5-1.0 ug/ml
IP: 1-2ug/500ug protein lysate
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD3e antibody to be titered up or down for optimal performance.
Properties
- PURIFICATION: Protein A purified CD3e antibody
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
- CONCENTRATION: 0.2 mg/mL
- STORAGE CONDITIONS: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: CD3E
- ADDITIONAL NAMES: T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T3E
- NCBI GENE ID NUMBER: 916
- USER NOTE: Optimal dilutions for each application to be determined by the researcher
Background
- This antibody recognizes the epsilon-chain of CD3 (CD3e), which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the Tcell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the Tcell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the Tcell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the Tcell surface. CD3 antigen is a highly specific marker for Tcells, and is present in majority of Tcell neoplasms.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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