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CD31 Antibody [PCM25-1]CD31 Antibody [PCM25-1]CD31 Antibody [PCM25-1]
CD31 Antibody [PCM25-1]
CD31 Antibody [PCM25-1]
CD31 Antibody [PCM25-1]

CD31 Antibody [PCM25-1]

CATALOG NUMBER: 33-863
  • Clonality: Monoclonal
  • Tested Applications: Flow, IF, IHC, WB
  • Host Species: Mouse
  • Species Reactivity: Human
  • Conjugate: Unconjugated
  • Size: 100 ug

Specifications

  • HOST SPECIES: Mouse
  • SPECIES REACTIVITY: Human
  • IMMUNOGEN: Recombinant human protein was used as the immunogen for this CD31 antibody.
  • CONJUGATE: Unconjugated
  • TESTED APPLICATIONS: Flow, IF, IHC, WB
  • APPLICATION NOTE: Western blot: 1-2 ug/ml
    Flow Cytometry: 0.5-1ug/10e6 cells
    IF: 1-2 ug/ml
    IHC (FFPE): 1-2 ug/ml for 30 minutes at RT (1)
    Prediluted format : incubate for 30 min at RT (2)
    Titering of the CD31 antibody may be required for optimal performance.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Properties

  • PURIFICATION: Protein G affinity chromatography
  • CLONALITY: Monoclonal
  • ISOTYPE: IgG1, k
  • PHYSICAL STATE: Liquid
  • BUFFER: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
  • CONCENTRATION: 0.2 mg/mL
  • STORAGE CONDITIONS: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.

Additional Info

  • NCBI OFFICIAL SYMBOL: PECAM1
  • ADDITIONAL NAMES: Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, CD31, PECAM1
  • NCBI GENE ID NUMBER: 5175
  • USER NOTE: Optimal dilutions for each application to be determined by the researcher

Background

  • CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. It is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [RefSeq]

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER: 33-863

  • Size: 100 ug
  • List Price: $555.00
Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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