Specifications
- HOST SPECIES: Mouse
- SPECIES REACTIVITY: Bovine, Human, Mouse, Pig, Sheep
- IMMUNOGEN: Stimulated human leukocytes were used as the immunogen for the CD28 antibody.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: Flow, Functional Assays, IF
- APPLICATION NOTE: Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Functional Activity (Order SAF formulation)
Optimal dilution of the CD28 antibody should be determined by the researcher.
Properties
- PURIFICATION: Protein G affinity chromatography
- CLONALITY: Monoclonal
- ISOTYPE: IgG2a, kappa
- PHYSICAL STATE: Liquid
- BUFFER: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
- CONCENTRATION: 0.2 mg/mL
- STORAGE CONDITIONS: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: CD28
- ADDITIONAL NAMES: CD4 Antibody [9H5A8] : CD4mut
- NCBI GENE ID NUMBER: 920
- USER NOTE: Optimal dilutions for each application to be determined by the researcher
Background
- Recognizes a glycoprotein of 44-88kDa, which is identified as CD28. It is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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