CD25 Antibody [3C7] (FITC)
CATALOG NUMBER: 98-706- Clonality: Monoclonal
- Tested Applications: Flow, ICC, IHC-Fr, IHC-P, IP, WB
- Host Species: Rat
- Species Reactivity: Mouse
- Conjugate: FITC
- Size: 0.5 mg
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- HOST SPECIES: Rat
- SPECIES REACTIVITY: Mouse
- IMMUNOGEN: IL-2 dependent BALB/c helper T cell clone HT2
- CONJUGATE: FITC
- TESTED APPLICATIONS: Flow, ICC, IHC-Fr, IHC-P, IP, WB
- APPLICATION NOTE: CD25 Antibody [3C7] for use in flow cytometry and immunohistochemistry assays.
- SPECIFICITY: CD25
Properties
- CLONALITY: Monoclonal
- ISOTYPE: IgG2b
- PHYSICAL STATE: Liquid
- BUFFER: Supplied in PBS/NaN3
- CONCENTRATION: 0.5 mg/mL
- STORAGE CONDITIONS: Store vial at 2-8°C
Additional Info
- NCBI OFFICIAL SYMBOL: Il2ra
- ADDITIONAL NAMES: IL-2Ralpha, p55, IL-2 receptor alpha chain, Ly-43, Tac, IL-2-RA
- Protein Accession Number: P01590
- NCBI GENE ID NUMBER: 16184
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity alpha chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail. CD25 is expressed on activated T cells, B cells, NK cells, and monocytes of all mouse strains tested. Expression of CD25 on activated T lymphocytes is transitory and endogenously regulated. CD25 is expressed on precursor B cells in bone marrow. Its expression is initiated by functional rearrangement and expression of IgM heavy chain genes and is down-regulated when immature B cells mature and express IgD. It is expressed at a higher level on CD4+CD8+ thymocytes. It is also expressed on cultured epidermal Langerhans cells. The biochemical features of murine CD25 have been characterized in detail. The 3C7 monoclonal antibody reacts with an epitope of CD25 which is distinct from that recognized by clone 7D4. 3C7, used in combination with 7D4 in culture, results in higher levels of inhibition of proliferation driven by IL-2 and generation of alloreactive CTL than either of these monoclonal antibodies alone.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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$35 Dry Ice Shipment fee may be required on some items.
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