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CD140 b Recombinant Protein
CD140 b Recombinant Protein

CD140 b Recombinant Protein

CATALOG NUMBER: 96-621
  • Tested Applications: WB

Specifications

  • SPECIES: Human
  • SOURCE SPECIES: HEK293 cells
  • RECOMBINANT PROTEIN SEQUENCE: Leu 33 - Phe 530
  • FUSION TAG: His Tag
  • TESTED APPLICATIONS: WB
  • APPLICATION NOTE: This recombinant protein can be used for WB. For research use only.
  • PREDICTED MOLECULAR WEIGHT: 57.0 kDa
  • BIOLOGICAL ACTIVITY: Measured by its ability to inhibit the biological activity of PDGFBB using NR6R-3T3 mouse fibroblast cells. The ED50 for this effect is typically 3-8μg/ml in the presence of 4 ng/mL rhPDGFBB.

Properties

  • PURITY: >95% as determined by SDS-PAGE.
  • PHYSICAL STATE: Lyophilized
  • BUFFER: PBS, pH7.4
  • STORAGE CONDITIONS: Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.

Additional Info

  • NCBI OFFICIAL SYMBOL: PDGFRB
  • ADDITIONAL NAMES: PDGFRB, CD140B, JTK12, PDGF-R-beta, PDGFR-2, PDGFR-beta, PDGFR-β
  • Protein Accession Number: AAH32224
  • NCBI GENE ID NUMBER: 5159

Background

  • Human platelet-derived growth factor receptor, beta polypeptide (PDGFRB) is also called J03278, M21616, CD140B, JTK12, PDGF-R-beta and PDGFR, is receptor that binds specifically to PDGFB and has a tyrosine-protein kinase activity. Is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor (PDGF) family. The PDGFR/PDGF system includes two receptors (PDGFRA and PDGFRB) and four ligands (A, B, C and D). The receptors PDGFRA and PDGFRB are related in sequence and both are members of the class III subtype of receptor tyrosine kinases (RTKs). Other class III RTKs are CSF1R, KIT and FLT3. PDGF binding induces receptor homo- and heterodimerization and signal transduction. The expression of the α and β receptors is independently regulated in various cell types. Recombinant soluble PDGFRB binds PDGF with high affinity and is potent PDGF antagonist. The ligands form either homo- or heterodimers (PDGF-AA, -AB, -BB, -CC, -DD). The four PDGFs are inactive in their monomeric forms. The PDGFs bind to the protein tyrosine kinase receptors PDGF receptor-α and -β. These two receptor isoforms dimerize upon binding the PDGF dimer, leading to three possible receptor combinations, namely -αα, -ββ and -αβ. PDGF-CC specifically interacts with PDGFR-αα and -αβ, but not with –ββ, and thereby resembles PDGF-AB. PDGF-DD binds to PDGFR-ββ with high affinity, and to PDGFR-αβ to a much lower extent and is regarded as PDGFR-ββ specific. PDGF-AA binds only to PDGFR-αα, while PDGF-BB is the only PDGF that can bind all three receptor combinations with high affinity.
  • 1: Chi, KD. et al., 1997, Oncogene 15: 1051-7.
  • 2: Golub TR. et al., 1994, Cell, 77 (2) : 307-316.
  • 3: Morerio, C. et al., 2004, Cancer Res. 64: 2649-51.

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER: 96-621

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