- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: Cbl antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human cbl.
The immunogen is located within amino acids 810 - 860 of Cbl.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, WB
- APPLICATION NOTE: Cbl antibody can be used for detection of cbl by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
- POSITIVE CONTROL 1: Cat. No. 1224 - Daudi Cell Lysate
- POSITIVE CONTROL 2: Cat. No. 1369 - Human Lymph node Tissue Lysate
- POSITIVE CONTROL 3: Cat. No. 11-521 - Human Lymphoid Tissue Tissue Slide
- PURIFICATION: Cbl Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: Cbl Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: Cbl antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- NCBI OFFICIAL SYMBOL: CBL
- ADDITIONAL NAMES: Cbl Antibody: CBL2, NSLL, C-CBL, RNF55, FRA11B, CBL2, E3 ubiquitin-protein ligase CBL, Casitas B-lineage lymphoma proto-oncogene
- Protein Accession Number: P22681
- PROTEIN GI NUMBER: 115855
- NCBI GENE ID NUMBER: 867
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
- Cbl Antibody: The mammalian cbl family of ubiquitin ligases consists of three homologs known as cbl (also known as c-Cbl), Cbl-B, and Cbl-3 which share highly conserved a tyrosine-kinase-binding domain, linker and RING finger domain in their amino-terminal halves. Similar to other E3 ubiquitin ligases, Cbl catalyzes the transfer of ubiquitin from an E2 or Ubc (ubiquitin-conjugating) enzyme to the e-amino group of a lysine residue of the substrate protein. Cbl acts to negatively regulate many types of cell-surface receptors, including the Syk protein tyrosine kinase family. Cbl is thought to be involved in T- and B-cell signaling, in addition to thymus development. Of the three known homologs in the cbl family, cbl antibody reacts specifically with cbl. Multiple isoforms of cbl have been reported.
- 1: Thien CBF and Langdon WY. C-Cbl and Cbl-b ubiquitin ligases: substrate diversity and the negative regulation of signaling responses. Biochem. J. 2005; 391:153-66
- 2: Weissman AM. Themes and variations on ubiquitylation. Nat. Rev. Mol. Cell Biol. 2001; 2:169-78.
- 3: Swaminathan G and Tsygankov AY. The Cbl family of proteins: ring leaders in regulation of cell signaling. J. Cell. Physiol. 2006; 209:21-43.
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page.
- Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
CATALOG NUMBER: 3967
- Size: 0.02 mg | 0.1 mg
- List Price: $89.00 – $395.00
$65 Standard Overnight flat rate for as many products shipped within the United States.
$35 Dry Ice Shipment fee may be required on some items.
Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.
Customize your order
Bulk Pricing Available
Custom Formulations: azide free, endotoxin testing & endotoxin removal.
Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.
Contact our Customer Care Team for more information
Custom Antibody Services
Speak to one of our custom antibody specialists to discuss your next custom antibody project.
New & Featured Products
HIV-1 p24 Antibody [10A2] (biotin)
CATALOG NUMBER: PM-7371-biotin
HIV-1 p24 Antibody [9D2F6] (biotin)
CATALOG NUMBER: PM-7369-biotin
HIV-1 p24 Antibody [8D7E11] (biotin)
CATALOG NUMBER: PM-7367-biotin