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Caspase-9 AntibodyCaspase-9 AntibodyCaspase-9 AntibodyCaspase-9 AntibodyCaspase-9 AntibodyCaspase-9 Antibody
Caspase-9 Antibody
Caspase-9 Antibody
Caspase-9 Antibody
Caspase-9 Antibody
Caspase-9 Antibody
Caspase-9 Antibody
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Caspase-9 Antibody

CATALOG NUMBER: 2071
  • Clonality: Polyclonal
  • Tested Applications: E, WB
  • Host Species: Rabbit
  • Species Reactivity: Human, Mouse, Rat
  • Conjugate: Unconjugated
  • Size: 0.02 mg | 0.1 mg

Specifications

  • HOST SPECIES: Rabbit
  • SPECIES REACTIVITY: Human, Mouse, Rat
  • IMMUNOGEN: Anti- Caspase 9 antibody (2071) was raised against a peptide corresponding to 16 amino acids near the amino terminus of human Caspase 9.
    The immunogen is located within amino acids 30-80 of Caspase 9.
  • CONJUGATE: Unconjugated
  • TESTED APPLICATIONS: E, WB
  • APPLICATION NOTE: WB: 0.5-2 μg/mL.
    Antibody validated: Western Blot in human, mouse and rat samples. All other applications and species not yet tested.
  • SPECIFICITY: It has no cross response to other members in caspase family.
  • POSITIVE CONTROL 1: Cat. No. 1201 - HeLa Cell Lysate
  • POSITIVE CONTROL 2: Cat. No. 1204 - K562 Cell Lysate
  • POSITIVE CONTROL 3: Cat. No. 1466 - Rat Spleen Lysate
  • PREDICTED MOLECULAR WEIGHT: Predicted: 46kD
    Observed: 46kD

Advanced Validation

  • VALIDATION:

    Independent Antibody Validation in Cell lines (Figure 1) shows similar Caspase 9 expression profile in human cell lines detected by two independent anti-Caspase 9 antibodies that recognize different epitopes, 2071 against N-terminus domain and 2073 against central domain.  Caspase 9 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies.

  • ISOFORMS: Human Caspase 9 has 4 isoforms, including isoform 1 (416aa, 46kD), isoform 2 (266aa, 30kD), isoform 3 (154aa, 17kD), and isoform 4 (333aa, 37kD). Mouse Caspase 9 has one isoform (454aa, 50kD) and rat Caspase 9 also has one isoform (454aa, 50kD). 2071 can detect human, mouse and rat.

Properties

  • PURIFICATION: Caspase-9 Antibody is affinity chromatography purified via peptide column.
  • CLONALITY: Polyclonal
  • ISOTYPE: IgG
  • PHYSICAL STATE: Liquid
  • BUFFER: Caspase-9 Antibody is supplied in PBS containing 0.02% sodium azide.
  • CONCENTRATION: 1 mg/ml
  • STORAGE CONDITIONS: Caspase-9 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

  • NCBI OFFICIAL SYMBOL: CASP9
  • ADDITIONAL NAMES: Caspase-9 Antibody: MCH6, APAF3, APAF-3, PPP1R56, ICE-LAP6, MCH6, Caspase-9, Apoptotic protease Mch-6, CASP-9
  • Protein Accession Number: P55211
  • PROTEIN GI NUMBER: 28558771
  • NCBI GENE ID NUMBER: 842
  • USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background

  • Caspase-9 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFα, TRAIL, anti-CD-95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues.
  • 1: Duan et al. J Biol Chem 1996;271:16720-4.
  • 2: Srinivasula et al. J Biol Chem 1996;271:27099-106.
  • 3: Li et al. Cell 1997;91:479-89.
  • 4: Pan et al. J Biol Chem 1998;273:5841-5 (RD1299).

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER: 2071

  • Size: 0.02 mg | 0.1 mg
  • List Price: $89.00 – $395.00
Datasheet
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