- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- IMMUNOGEN: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CAPN10.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, WB
- APPLICATION NOTE: CAPN10 antibody can be used for detection of CAPN10 by ELISA at 1:1562500. CAPN10 antibody can be used for detection of CAPN10 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
- POSITIVE CONTROL 1: HT1080 Cell Lysate
- PREDICTED MOLECULAR WEIGHT: 58 kDa
- PURIFICATION: Antibody is purified by peptide affinity chromatography method.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: For short periods of storage (days) store at 4°C. For longer periods of storage, store CAPN10 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
- NCBI OFFICIAL SYMBOL: CAPN10
- ADDITIONAL NAMES: CAPN10, CANP10, NIDDM1
- Protein Accession Number: NP_075573
- PROTEIN GI NUMBER: 89353291
- NCBI GENE ID NUMBER: 11132
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
- CAPN10 is the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large catalytic subunit has four domains: domain I, the N-terminal regulatory domain that is processed upon calpain activation; domain II, the protease domain; domain III, a linker domain of unknown function; and domain IV, the calmodulin-like calcium-binding domain. This gene encodes a large subunit. It is an atypical calpain in that it lacks the calmodulin-like calcium-binding domain and instead has a divergent C-terminal domain. It is similar in organization to calpains 5 and 6. This gene is associated with type 2 or non-insulin-dependent diabetes mellitus (NIDDM) and located within the NIDDM1 region. Multiple alternative transcript variants encoding different isoforms have been described for this gene.
- 1: Kifagi, C., (2008) Diabetes Metab. 34 (3), 273-278.
- FOR RESEARCH USE ONLY
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- Disclaimer: This product is for research use only.
CATALOG NUMBER: 26-646
- Size: 100 ul
- List Price: $430.00
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