Close Hamburger Menu Close Hamburger Menu
Home / Human / C-RAF (phospho Ser642) Antibody
C-RAF (phospho Ser642) Antibody
C-RAF (phospho Ser642) Antibody

C-RAF (phospho Ser642) Antibody

CATALOG NUMBER: 79-935
  • Clonality: Polyclonal
  • Tested Applications: WB
  • Host Species: Rabbit
  • Species Reactivity: Human, Mouse, Rat
  • Conjugate: Unconjugated
  • Size: 0.1 mg

Specifications

  • HOST SPECIES: Rabbit
  • SPECIES REACTIVITY: Human, Mouse, Rat
  • IMMUNOGEN: C-RAF (phospho Ser642) antibody was raised against a peptide sequence around phosphorylation site of Serine 642 (T-T-S (p)-P-R) derived from Human C-RAF.
  • CONJUGATE: Unconjugated
  • TESTED APPLICATIONS: WB
  • APPLICATION NOTE: Western Blot: 1:500~1:1000
  • SPECIFICITY: The antibody detects endogenous levels of C-RAF only when phosphorylated at serine 642.
  • PREDICTED MOLECULAR WEIGHT: 74 kDa

Properties

  • PURIFICATION: Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • CLONALITY: Polyclonal
  • ISOTYPE: IgG
  • PHYSICAL STATE: Liquid
  • BUFFER: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • CONCENTRATION: 1 mg/mL
  • STORAGE CONDITIONS: Store antibody at -20°C for up to one year.

Additional Info

  • NCBI OFFICIAL SYMBOL: RAF1
  • ADDITIONAL NAMES: C-RAF, C-Raf, CRAF, RAF-1
  • Protein Accession Number: NP_002871.1
  • PROTEIN GI NUMBER: 4506401
  • NCBI GENE ID NUMBER: 5894

Background

  • Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.
  • 1: Bonner T.I., Nucleic Acids Res. 14:1009-1015(1986).
  • 2: Ota T., Nat. Genet. 36:40-45(2004).
  • 3: Mural R.J., Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER: 79-935

  • Size: 0.1 mg
  • List Price: $335.00
Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services 

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products
HIV-1 p24 Antibody [10A2] (biotin)
HIV-1 p24 Antibody [10A2] (biotin)

CATALOG NUMBER: PM-7371-biotin

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [8D7E11] (biotin)
HIV-1 p24 Antibody [8D7E11] (biotin)

CATALOG NUMBER: PM-7367-biotin