- Trial Size
BRUCE Antibody
CATALOG NUMBER: 3309- Clonality: Polyclonal
- Tested Applications: E, IHC-P
- Host Species: Rabbit
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Mouse: (100%)
- IMMUNOGEN: BRUCE antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human BRUCE.
The immunogen is located within amino acids 4760 - 4810 of BRUCE. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IHC-P
- APPLICATION NOTE: BRUCE antibody can be used for detection of BRUCE by immunohistochemistry at 5 μg/mL.
Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested. - SPECIFICITY: BRUCE antibody is human specific. Multiple isoforms of BRUCE are known to exist.
- POSITIVE CONTROL 1: Cat. No. 11-801 - Human Small Intestine Tissue Slide
- PREDICTED MOLECULAR WEIGHT: 534 kDa
Properties
- PURIFICATION: BRUCE Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: BRUCE Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: BRUCE antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: BIRC6
- ADDITIONAL NAMES: BRUCE Antibody: BRUCE, APOLLON, KIAA1289, Baculoviral IAP repeat-containing protein 6, BIR repeat-containing ubiquitin-conjugating enzyme, BRUCE
- Protein Accession Number: NP_057336
- PROTEIN GI NUMBER: 153792694
- NCBI GENE ID NUMBER: 57448
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- BRUCE Antibody: Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and can be prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. BRUCE, also known as BIRC6, is an IAP family member protein with a BIR (baculoviral inhibition of apoptosis protein repeat) domain and a UBCc (ubiquitin-conjugating enzyme E2, catalytic) domain. BRUCE regulates p53 and the mitochondrial pathway of apoptosis by facilitating the degradation of apoptotic proteins such as Caspase-9 and SMAC by ubiquitination.
- 1: Schimmer AD. Inhibitor of apoptosis proteins: translating basic knowledge into clinical practice. Cancer Res. 2004; 64:7183-90.
- 2: Chen Z, Naito M, Hori S, et al. A human IAP-gamily gene, apollon, expressed in human brain cancer cells. Biochem. Biophys. Res. Commun. 1999; 264:847-54
- 3: Hao Y, Sekine K, Kawabata A, et al. Apollon ubiquitinates SMAC and Caspase-9, and has an essential cytoprotection function. Nat. Cell Biol. 2004; 6:849-60.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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