- Trial Size
Bora Antibody
CATALOG NUMBER: 5107- Clonality: Polyclonal
- Tested Applications: ELISA, IF, IHC-P, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse, Rat
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: Bora antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human Bora.
The immunogen is located within amino acids 410 - 460 of Bora. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
- APPLICATION NOTE: Bora antibody can be used for detection of Bora by Western blot at 1-2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. - POSITIVE CONTROL 1: Cat. No. 1403 - Mouse Brain Tissue Lysate
Properties
- PURIFICATION: Bora Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: Bora Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: Bora antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: BORA
- ADDITIONAL NAMES: Bora Antibody: C13orf34, C13orf34, Protein aurora borealis, HsBora
- Protein Accession Number: Q6PGQ7
- PROTEIN GI NUMBER: 74737659
- NCBI GENE ID NUMBER: 79866
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Bora Antibody: Bora (Protein aurora borealis) is a key activator of Aurora Related Protein Kinase A (ARK-1), which is a centrosome-associated serine/threonine kinase that regulates centrosome maturation, bipolar spindle assembly and chromosome segregation during mitosis. Bora is localized to the nucleus until mitosis is initiated, then translocates to the cytoplasm in a Cdc2 dependent manner. Activation of Cdc2 initiates the release of Bora into the cytoplasm where it can bind and activate ARK-1. PLK1 (polo-like kinase-1) interacts with Bora to control the accessibility of its activation loop for phosphorylation and activation by ARK-1. Bora and ARK-1 cooperatively activate PLK1 and control mitotic entry. Bora mutants result in multipolar spindles in mitosis identical to those observed when ARK-1 function is blocked. Thus, the ARK1-Bora-PLK1 regulatory circuit in mammalian cells elucidates a key mechanism in cell cycle regulation. At least three isoforms of Bora are known to exist.
- 1: Berdnik D and Knoblich JA. Drosophila Aurora A is required for centrosome maturation and Actin-dependent asymmetric protein localization during mitosis. Curr. Biol.2002; 12:640-647.
- 2: Wiese C and O'Brien LL. What's so Bor(a)ing about Aurora A activation? Dev. Cell2006; 11:133-134.
- 3: Hutterer A, Berdnik D, Wirtz-Peitz F, et al. Mitotic activation of the kinase Aurora A requires its binding partner Bora. Dev. Cell2006; 11:147-157.
- 4: Fu J, Bian M, Jiang Q, et al.. Roles of Aurora kinases in mitosis and tumorigenesis. Mol. Cancer Res.2007; 5:1-10.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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