Specifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse
- IMMUNOGEN: This BNIP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152-187 amino acids from human BNIP3.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: IF, IHC-P, WB
- APPLICATION NOTE: For IF starting dilution is: 1:500
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100 - PREDICTED MOLECULAR WEIGHT: 22 kDa
Properties
- PURIFICATION: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
- CLONALITY: Polyclonal
- ISOTYPE: Rabbit Ig
- PHYSICAL STATE: Liquid
- BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: BNIP3
- ADDITIONAL NAMES: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3, BNIP3, NIP3
- Protein Accession Number: Q12983
- PROTEIN GI NUMBER: 20532402
- NCBI GENE ID NUMBER: 664
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- NIP3 is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. NIP3 contains a BH3 domain and a transmembrane domain, which have been associated with pro-apoptotic function. The dimeric mitochondrial protein is known to induce apoptosis, even in the presence of BCL2.
- 1: References for protein:
- 2: Kothari, S., et al., Oncogene 22(30):4734-4744 (2003).
- 3: Lee, S.M., et al., Life Sci. 71(19):2267-2277 (2002).
- 4: Ray, R., et al., J. Biol. Chem. 275(2):1439-1448 (2000).
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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