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Home / ELISA / bNGF Antibody [M85-2B]
bNGF Antibody [M85-2B]bNGF Antibody [M85-2B]
bNGF Antibody [M85-2B]
bNGF Antibody [M85-2B]

bNGF Antibody [M85-2B]

CATALOG NUMBER: 38-239
  • Clonality: Monoclonal
  • Tested Applications: ELISA, Neutralization Assay, WB
  • Host Species: Mouse
  • Species Reactivity: Human
  • Conjugate: Unconjugated
  • Size: 0.5 mg

Specifications

  • HOST SPECIES: Mouse
  • SPECIES REACTIVITY: Human
  • IMMUNOGEN: beta-NGF antibody is produced in mice using highly pure (>98%) recombinant human beta-NGF as the immunizing antigen.
  • CONJUGATE: Unconjugated
  • TESTED APPLICATIONS: ELISA, Neutralization Assay, WB
  • APPLICATION NOTE: Neutralization:
    To yield one-half maximal inhibition [ND50] of the biological activity of hB-NGF (10.0 ng/mL), a concentration of 2.7 - 4.0 μg/mL of this antibody is required.
    ELISA:
    In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 200 pg/mL of recombinant human β-NGF when used with our biotinylated antigen affinity purified anti-human b-NGF as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.
    Western Blot:
    To detect hβ-NGF by Western Blot analysis this antibody can be used at a concentration of 4.0-8.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hβ-NGF is 4.0-8.0 ng/lane, under reducing or non-reducing conditions.

Properties

  • PURIFICATION: This IgG2bK antibody was purified from cell culture by Protein G affinity chromatography.
  • CLONALITY: Monoclonal
  • PHYSICAL STATE: Lyophilized
  • CONCENTRATION: batch dependent
  • STORAGE CONDITIONS: beta-NGF lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.

Additional Info

  • NCBI OFFICIAL SYMBOL: NGF
  • ADDITIONAL NAMES: Nerve growth factor, NGFB, HSAN5, Beta-NGF, MGC161426, MGC161428 , Beta-nerve growth factorBeta-NGF
  • Protein Accession Number: P01138
  • PROTEIN GI NUMBER: 90110037
  • NCBI GENE ID NUMBER: 4803
  • USER NOTE: Centrifuge vial prior to opening.

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: This product is for research use only.

CATALOG NUMBER: 38-239

  • Size: 0.5 mg
  • List Price: $335.00
Datasheet
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