BMP1 Antibody
CATALOG NUMBER: 43-642- Clonality: Polyclonal
- Tested Applications: ELISA, IHC, WB
- Host Species: Goat
- Species Reactivity: Human, Mouse, Rat
- Conjugate: Unconjugated
- Size: 0.1 mg
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- HOST SPECIES: Goat
- SPECIES REACTIVITY: Human, Mouse, Rat
- HOMOLOGY: Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
- IMMUNOGEN: The immunogen for this antibody is: C-DTIVPKYEVNGVK
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, IHC, WB
- APPLICATION NOTE: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 110kDa band observed in Human Heart and Human Kidney lysates and approx 100kDa band observed in Rat Heart lysates and in cell lysates of cell line NIH3T3 (calculated MW of 111kDa according to Human NP_006120.1, 97.7kDa according to Mouse XP_011243231.1, and 93.5kDa according to Rat XP_006252347.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Pig Heart and Kidney for this particular batch.Immunohistochemistry:Paraffin embedded Human Brain (Cortex) and Placenta. Recommended concentration: 5ug/ml.
- SPECIFICITY: This antibody is expected to recognize both reported isoforms (NP_001190.1; NP_006120.1).
- PREDICTED MOLECULAR WEIGHT: Approx 110kDa
Properties
- PURIFICATION: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
- CONCENTRATION: 500 ug/mL
- STORAGE CONDITIONS: Aliquot and store at -20°C. Minimize freezing and thawing.
Additional Info
- NCBI OFFICIAL SYMBOL: BMP1
- ADDITIONAL NAMES: BMP1, bone morphogenetic protein 1, OI13, PCOLC, PCP, PCP2, TLD, mammalian tolloid protein, procollagen C-endopeptidase, procollagen C-proteinase
- Protein Accession Number: NP_001190.1,NP_006120.1
- PROTEIN GI NUMBER: 13124688
- NCBI GENE ID NUMBER: 649
Background
- 1: Wermter C, Höwel M, Hintze V, Bombosch B, Aufenvenne K, Yiallouros I, Stöcker W.The protease domain of procollagen C-proteinase (BMP1) lacks substrate selectivity, which is conferred by non-proteolytic domains.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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