BLNK Antibody
CATALOG NUMBER: 45-025- Clonality: Polyclonal
- Tested Applications: ELISA, Flow, IF, WB
- Host Species: Goat
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 0.1 mg
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- HOST SPECIES: Goat
- SPECIES REACTIVITY: Human
- HOMOLOGY: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
- IMMUNOGEN: The immunogen for this antibody is: C-KDSTRLKYAVKVS
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, Flow, IF, WB
- APPLICATION NOTE: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 70-75kDa band observed in lysates of cell line Daudi (calculated MW of 50.5kDa according to NP_037446.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration 0.3-1ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the membranes of HepG2 cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of Daudi cells. Recommended concentration: 10ug/ml.
- SPECIFICITY: This antibody is expected to recognize all reported isoforms.
- POSITIVE CONTROL 1: Cat. No. 1224 - Daudi Cell Lysate
Properties
- PURIFICATION: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
- CONCENTRATION: 500 ug/mL
- STORAGE CONDITIONS: Aliquot and store at -20°C. Minimize freezing and thawing.
Additional Info
- NCBI OFFICIAL SYMBOL: BLNK
- ADDITIONAL NAMES: BLNK, SLP-65, B-cell linker, BASH, BLNK-s, Ly57, MGC111051, SLP65, B cell linker protein, B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, OTTHUMP00000020167, Src homology 2 domain-containing leukocyte protein of 65 kDa, BLNK-S, LY57, OTTHUMP00000020168
- Protein Accession Number: NP_037446.1,NP_001107566.1,NP_001245369.1,NP_001245370.1,NP_001245371.1
- PROTEIN GI NUMBER: 7019535
- NCBI GENE ID NUMBER: 29760
Background
- 1: Fu C, Turck CW, Kurosaki T, Chan AC. BLNK: a central linker protein in B cell activation. Immunity. 1998 Jul;9(1):93-103.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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